Loading...
Please wait, while we are loading the content...
Similar Documents
Differential Requirement for CD4 Help in the Development of an Antigen-Specific CD8+ T Cell Response Depending on the Route of Immunization
| Content Provider | Scilit |
|---|---|
| Author | Bour, Hélène Horvath, Clotilde Lurquin, Christophe Cerottini, Jean-Charles MacDonald, H. Robson |
| Copyright Year | 1998 |
| Description | Previous studies in our laboratory have shown that DBA/2 mice injected i.p. with syngeneic P815 tumor cells transfected with the HLA-CW3 gene (P815-CW3) showed a dramatic expansion of activated $CD8^{+}CD62L^{−}$ T cells expressing exclusively the Vβ10 segment. We have used this model to study the regulatory mechanisms involved in the development of the CW3-specific $CD8^{+}$ response, with respect to different routes of immunization. Whereas both intradermal (i.d.) and i.p. immunization of DBA/2 mice with P815-CW3 cells led to a strong expansion of $CD8^{+}CD62L^{−}Vβ10^{+}$ cells, only the i.d. route allowed this expansion after immunization with P815 cells transfected with a minigene coding for the antigenic epitope CW3 170–179 (P815 miniCW3). Furthermore, depletion of $CD4^{+}$ T cells in vivo completely abolished the specific response of $CD8^{+}CD62L^{−}Vβ10^{+}$ cells and prevented the rejection of P815-CW3 tumor cells injected i.p, whereas it did not affect $CD8^{+}CD62L^{−}Vβ10^{+}$ cell expansion after i.d. immunization with either P815-CW3 or P815 miniCW3. Finally, the CW3-specific $CD8^{+}$ memory response was identical whether or not $CD4^{+}$ T cells were depleted during the primary response. Collectively, these results suggest that the $CD8^{+}$ T cell response to P815-CW3 tumor cells injected i.p. is strictly dependent upon recognition of a helper epitope by $CD4^{+}$ T cells, whereas no such requirement is observed for i.d. injection. |
| Ending Page | 5529 |
| Page Count | 8 |
| Starting Page | 5522 |
| ISSN | 00221767 |
| e-ISSN | 15506606 |
| DOI | 10.4049/jimmunol.160.11.5522 |
| Journal | The Journal of Immunology |
| Issue Number | 11 |
| Volume Number | 160 |
| Language | English |
| Publisher | The American Association of Immunologists |
| Publisher Date | 1998-06-01 |
| Access Restriction | Open |
| Subject Keyword | P815 Cw3 Cell Response Cells Transfected Cw3 Specific Cd8 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
