ES-62, an Immunomodulator Secreted by Filarial Nematodes, Suppresses Clonal Expansion and Modifies Effector Function of Heterologous Antigen-Specific T Cells In Vivo

Content Provider	Scilit
Author	Marshall, Fraser A. Grierson, Angela M. Garside, Paul Harnett, William Harnett, Margaret M.
Copyright Year	2005
Description	ES-62 is a phosphorylcholine-containing glycoprotein secreted by filarial nematodes, which has previously been shown to possess a range of immunomodulatory capabilities. We now show, using a $CD4^{+}$ transgenic TCR T cell adoptive transfer system, that ES-62 can modulate heterologous Ag (OVA)-specific responses in vivo. Thus, in contrast to the mixed IgG1-IgG2a response observed in control animals, ES-62-treated mice exhibited a Th2-biased IgG Ab response as evidenced by stable enhancement of anti-OVA IgG1 production and a profound inhibition of anti-OVA IgG2a. Consistent with this, Ag-specific IFN-γ produced was suppressed by pre-exposure to ES-62 when T cells were rechallenged ex vivo. However, the response observed was not classical Th2, because although Ag-specific IL-5 production was enhanced by pre-exposure to ES-62, IL-13, and IL-4 were inhibited when T cells were rechallenged ex vivo. Moreover, such T cells produced lower levels of IL-2 and proliferated less upon Ag rechallenge ex vivo. Finally, pre-exposure to ES-62 inhibited the clonal expansion of the transferred Ag-specific $CD4^{+}$ T cells and altered the functional response of such T cells in vivo, by modulating the kinetics and reducing the extent of their migration into B cell follicles.
Ending Page	5826
Page Count	10
Starting Page	5817
ISSN	00221767
e-ISSN	15506606
DOI	10.4049/jimmunol.175.9.5817
Journal	The Journal of Immunology
Issue Number	9
Volume Number	175
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2005-11-01
Access Restriction	Open
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology