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Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy
| Content Provider | Scilit |
|---|---|
| Author | Freudiger, Christian W. Min, Wei Saar, Brian G. Lu, Sijia Holtom, Gary R. He, Chengwei Tsai, Jason C. Kang, Jing X. |
| Editor | Xie, X. Sunney |
| Copyright Year | 2008 |
| Description | Label-free chemical contrast is highly desirable in biomedical imaging. Spontaneous Raman microscopy provides specific vibrational signatures of chemical bonds, but is often hindered by low sensitivity. Here we report a three-dimensional multiphoton vibrational imaging technique based on stimulated Raman scattering (SRS). The sensitivity of SRS imaging is significantly greater than that of spontaneous Raman microscopy, which is achieved by implementing high-frequency (megahertz) phase-sensitive detection. SRS microscopy has a major advantage over previous coherent Raman techniques in that it offers background-free and readily interpretable chemical contrast. We show a variety of biomedical applications, such as differentiating distributions of omega-3 fatty acids and saturated lipids in living cells, imaging of brain and skin tissues based on intrinsic lipid contrast, and monitoring drug delivery through the epidermis. |
| Related Links | http://science.sciencemag.org/content/322/5909/1857.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3576036/pdf https://science.sciencemag.org/content/sci/322/5909/1857.full.pdf |
| Ending Page | 1861 |
| Page Count | 5 |
| Starting Page | 1857 |
| ISSN | 00368075 |
| e-ISSN | 10959203 |
| DOI | 10.1126/science.1165758 |
| Journal | Science |
| Issue Number | 5909 |
| Volume Number | 322 |
| Language | English |
| Publisher | American Association for the Advancement of Science (AAAS) |
| Publisher Date | 2008-12-19 |
| Access Restriction | Open |
| Subject Keyword | Analytical Chemistry Three Dimensional Stimulated Raman Scattering Live Cell Imaging Biomedical Imaging |
| Content Type | Text |
| Resource Type | Article |
| Subject | History and Philosophy of Science |
