NDLI: Enzymatic hydrolysis of cassava starch for production of bioethanol with a colombian wild yeast strain

Content Provider	Scientific Electronic Library Online (SciELO)
Author	Ruiz, Mónica I. Sanchez, Clara I. Torrres, Rodrigo G. Molina, Daniel R.
Abstract	Enzymatic hydrolysis of cassava starch for producing glucose syrups was evaluated using alpha-amylase from Bacillus licheniformis and glucoamylase from Aspergillus niger. Moreover, an enzyme mixture of α-amylase from Aspergillus kawachi and glucoamylase from Aspergillus niger was tested. Enzyme conditions for starch hydrolysis were optimized by a factorial experimental design (3³×2) using as variables substrate concentration, enzyme/substrate ratio and time reaction. Optimal enzyme reactions with 100 g of starch per L were: α-amylase at pH 5.0, 80 °C and enzyme dosage of 130.5 U g-1 of starch; and glucoamylase, pH 4.5, 70 °C and enzyme dosage of 81.5 U g-1 of starch. Additionally, optimal conditions for the enzymatic mixture were pH 4.5, 46 °C, and enzyme dosage of 16.4 U g-1 of starch. Finally, alcohol production using glucose syrups from enzymatically-hydrolyzed starch was carried out with a wild strain of Candida sp isolated from sugar cane juice, obtaining volumetric ethanol productivities around 1.8-3.2 g L-1h-1.
Related Links	http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0103-50532011001200014&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011001200014&lng=en&nrm=iso
Starting Page	2337
Ending Page	2343
Page Count	7
File Format	PDF
ISSN	01035053
DOI	10.1590/S0103-50532011001200014
Journal	Journal of the Brazilian Chemical Society
Issue Number	12
Volume Number	22
Language	English
Publisher	Sociedade Brasileira de Química
Publisher Date	2011-12-01
Publisher Institution	Universidad Industrial de Santander Facultad de Ciencias Facultad de Salud Escuela de Química Universidad Industrial de Santander Facultad de Salud Escuela de Bacteriología y Laboratorio Clínico
Publisher Place	Colombia
Access Restriction	Open
Subject Keyword	Cassava starch Enzymatic hydrolysis Amylases Bioethanol
Content Type	Text
Resource Type	Article
Subject	Chemistry

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