NDLI: Predominance of Leishmania major and rare occurrence of Leishmania tropica with haplotype variability at the center of Iran

Content Provider	Scientific Electronic Library Online (SciELO)
Author	Zahirnia, Amir Hossein Bordbar, Ali Ebrahimi, Sahar Spotin, Adel Mohammadi, Somayeh Ghafari, Seyedeh Maryam Ahmadvand, Setareh Jabbari, Negar Esmaeili Rastaghi, Ahmad Reza Parvizi, Parviz
Abstract	ABSTRACT Background Leishmania major is a causative agent of zoonotic cutaneous leishmaniasis in the center of Iran, Abarkouh district. Molecular characterization and precise incrimination of Leishmania species was carried out to perform controlling measurements and to design treatment programs for zoonotic cutaneous leishmaniasis. Methods All smears isolated from ulcers of suspected patients were examined under a light microscope and graded for amastigotes frequency. Extraction of DNA, PCR, RFLP and sequencing of ITS-rDNA genotype were done to increase the efficacy of Leishmania parasites identification at their species-specific level and to detect any Leishmania infections within. Results Humans were found to be infected with L. major with high infection frequency and also Leishmania tropica was identified with low occurrence for the first time as non-native species using molecular analyses. The rates of infections was considerable with microscopic observation (n= 65, 73%) out of 89 smears prepared from suspected patients. Molecular analyses showed that the density of L. major was significantly higher (n= 48, 53.93%) than L. tropica (n= 4, 4.49%) (Mann-Whitney U test: p< 0.05) and two samples (2.25%) remained ambiguous after several sequencing. L. major did not have diversity with two common haplotypes but L. tropica were found to exhibit high diversity with three novel haplotypes. Conclusion L. major was considered the causative agent of leishmaniasis in the region, but the identification of a non-native L. tropica revealed the importance of further isolation of Leishmania parasites following molecular analyses and confirmation, and also revealed the importance of further isolation of Leishmania parasites from patients of the field areas who do not have easily access to health care centers for specialized treatment strategies.
Related Links	http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1413-86702018000400278&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000400278&lng=en&nrm=iso
Starting Page	278
Ending Page	287
Page Count	10
File Format	PDF
ISSN	14138670
DOI	10.1016/j.bjid.2018.07.005
Journal	Brazilian Journal of Infectious Diseases
Issue Number	4
Volume Number	22
Language	English
Publisher	Brazilian Society of Infectious Diseases
Publisher Date	2018-08-01
Access Restriction	Open
Subject Keyword	Leishmaniasis Leishmania major Leishmania tropica Nuclear gene Iran
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology (medical)

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