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Study on proanthocyanidins crosslinked collagen membrane for guided bone tissue regeneration
| Content Provider | SAGE Publishing |
|---|---|
| Author | Yang, Hongfa Liu, Wai-Ching Liu, Xinrui Li, Yunqian Lin, Chingpo Lin, Yu-Min Wang, An-Ni Nguyen, Thu-Trang Lin, Yu-Chien Chung, Ren-Jei |
| Copyright Year | 2021 |
| Abstract | The goal of this study is to understand the ability of a newly developed barrier membrane to enhance bone tissue regeneration. Here in this study we present the in vitro characterization of the barrier membrane made from type I collagen and crosslinked by oligomeric proanthocyanidins (OPCs). The effects of the membrane (P-C film) on cell cycle, proliferation, alkaline phosphatase activity, and mineralization were evaluated using the human osteoblast cell line MG-63, while the barrier ability was examined using MG-63 cells, as well as the human skin fibroblast cell line WS-1. The pore size is one of the factors that plays a key role in tissue regeneration, therefore, we evaluated the pore size of the membrane using a capillary flow porometer. Our results showed that the mean pore size of the P-C film was approximately 7–9 µm, the size known to inhibit cell migration across the membrane. The P-C film also demonstrated excellent cell viability and good biocompatibility, since the cell number increased with time, with MG-63 cells proliferating faster on the P-C film than in the cell culture flask. Furthermore, the P-C film promoted osteoblast differentiation, resulting in higher alkaline phosphatase activity and mineralization. Therefore, our results suggest that this P-C film has a great potential to be used in guided bone regeneration during periodontal regeneration and bone tissue engineering. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/22808000211005379?download=true |
| ISSN | 22808000 |
| Volume Number | 19 |
| Journal | Journal of Applied Biomaterials & Functional Materials (JBF) |
| e-ISSN | 22808000 |
| DOI | 10.1177/22808000211005379 |
| Language | English |
| Publisher | Sage Publications UK |
| Publisher Date | 2021-03-30 |
| Publisher Place | London |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2021 |
| Subject Keyword | barrier membrane oligomeric proanthocyanidins Type I collagen guided bone regeneration |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biomaterials Biophysics Bioengineering Biomedical Engineering |
