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Osteogenic potential of mesenchymal stem cells from rat mandible to regenerate critical sized calvarial defect
| Content Provider | SAGE Publishing |
|---|---|
| Author | Lee, Dong Joon Kwon, Jane Current, Luke Yoon, Kun Zalal, Rahim Hu, Xiangxiang Xue, Peng Ko, Ching-Chang |
| Copyright Year | 2019 |
| Abstract | Although bone marrow–derived mesenchymal stem cells (MSCs) have been extensively explored in bone tissue engineering, only few studies using mesenchymal stem cells from mandible (M-MSCs) have been reported. However, mesenchymal stem cells from mandible have the potential to be as effective as femur-derived mesenchymal stem cells (F-MSCs) in regenerating bone, especially in the orofacial regions, which share embryonic origin, proximity, and accessibility. M-MSCs were isolated and characterized using mesenchymal stem cell–specific markers, colony forming assay, and multi-potential differentiation. In vitro osteogenic potential, including proliferation, osteogenic gene expression, alkaline phosphatase activity, and mineralization, was examined and compared. Furthermore, in vivo bone formations of F-MSCs and M-MSCs in rat critical sized defect were evaluated using microCT and histology. M-MSCs from rat could be successfully isolated and expanded while preserving their MSC’s characteristics. M-MSCs demonstrated a comparable proliferation and mineralization potentials and in vivo bone formation as F-MSCs. M-MSCs is a promising cell source candidate for craniofacial bone tissue engineering. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/2041731419830427?download=true |
| ISSN | 20417314 |
| Volume Number | 10 |
| Journal | Journal of Tissue Engineering (TEJ) |
| e-ISSN | 20417314 |
| DOI | 10.1177/2041731419830427 |
| Language | English |
| Publisher | Sage Publications UK |
| Publisher Date | 2019-03-12 |
| Publisher Place | London |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2019 |
| Subject Keyword | Mesenchymal stem cells from mandible mandible critical sized defect craniofacial bone tissue engineering |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biomaterials Biomedical Engineering |
