Loading...
Please wait, while we are loading the content...
Antioxidant and Antitumorigenic Activities of the Standardized Water Extract From Fruit of Terminalia chebula Retz. var. chebula
| Content Provider | SAGE Publishing |
|---|---|
| Author | Takuathung, Mingkwan Na Wongnoppavich, Ariyaphong Jaijoy, Kanjana Soonthornchareonnon, Noppamas Sireeratawong, Seewaboon |
| Copyright Year | 2023 |
| Abstract | Terminalia chebula Retz. var. chebula (T chebula) has been traditionally used as a crude drug for treating various diseases, including skin disorders. This study aimed to investigate the potential antioxidant effects and antitumorigenic activity of an aqueous extract from the fruit of T chebula.MethodsThe extract's total phenolic content and antioxidant activity were measured using Folin-Ciocalteu, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) tests, respectively. The 2 ´,7 ´-dichlorodihydrofluorescin diacetate assay was employed to evaluate the extract's ability to reduce intracellular reactive oxygen species (ROS) in U-937 human monocytic cell lines which had been treated with hydrogen peroxide for 30 min. Male ICR mice (n = 30, 5 groups) were used to investigate the influence of T chebula extract on dimethylbenz[a]anthracene (DMBA)/12-O-tetradecanoylphorbol 13-acetate (TPA)-induced carcinogenesis. After 20 weeks of treatment with T chebula extract, tumor incidence, size, and number were assessed. The major phenolic compounds in the extract were determined using high-performance liquid chromatography. Statistical analysis was performed using ANOVA followed by post hoc least significant difference test.ResultsT chebula extract effectively reduced intracellular ROS and scavenged antioxidants in vitro. The IC50 values of the extract as measured by DPPH and FRAP assays were 109.0 ± 14.5 μg/mL and 2.39 ± 0.17 μg/mL, respectively. Moreover, 4 mg of T chebula extract significantly decreased the incidence, volume, and number of tumors in DMBA/TPA-induced skin tumorigenesis in mice.ConclusionT chebula extract effectively reduced intracellular ROS, and significantly decreased the incidence, volume, and number of tumors in DMBA/TPA-induced skin tumorigenesis in mice. These findings suggest that T chebula extract could be a promising therapeutic agent for skin tumors and other oxidative stress-related diseases. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/1934578X231176925?download=true |
| ISSN | 1934578X |
| Issue Number | 6 |
| Volume Number | 18 |
| Journal | Natural Product Communications (NPX) |
| e-ISSN | 15559475 |
| DOI | 10.1177/1934578X231176925 |
| Language | English |
| Publisher | Sage Publications CA |
| Publisher Date | 2023-06-12 |
| Publisher Place | Los Angeles |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2023 |
| Subject Keyword | antioxidant antitumorigenesis reactive oxygen species Terminalia chebula flavonoids |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Drug Discovery Pharmacology Complementary and Alternative Medicine |
