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An Alternative Cuprizone-Induced Demyelination and Remyelination Mouse Model
| Content Provider | SAGE Publishing |
|---|---|
| Author | Zhen, Wei Liu, Ailian Lu, Jiaqi Zhang, Wandong Tattersall, David Wang, Jianfei |
| Copyright Year | 2017 |
| Abstract | The cuprizone model is a well-established and investigated paradigm to study demyelination and remyelination in rodents. Cuprizone is usually administrated by mixing in the powdered or pelleted rodent chow. However, since cuprizone is sensitive to the environment and the consumption of it varies between different animals, the major issue is the discrepancy in demyelination of the animals. This study reports the development of the cuprizone model by gavage administrations in mice. Following testing a series of doses of cuprizone, 400 mg/kg/day was found to be the best dosage to induce dramatic and consistent demyelination after 5 weeks of administration; while remyelination quickly occurred after 9 days of cuprizone withdrawal. The advantage of this alternative model is that the consumption of cuprizone could be well controlled, and the mice were exposed to the same dose of cuprizone. Thus, the variation in demyelination was minimized. This alternative cuprizone dosing regime minimizes the interanimal variability on demyelination and hence provides a consistent model for pharmacological evaluations, in addition to reducing the number of animals used in the experiments. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/1759091417725174?download=true |
| ISSN | 17590914 |
| Issue Number | 4 |
| Volume Number | 9 |
| Journal | ASN Neuro (ASN) |
| e-ISSN | 17590914 |
| DOI | 10.1177/1759091417725174 |
| Language | English |
| Publisher | Sage Publications CA |
| Publisher Date | 2017-08-25 |
| Publisher Place | Los Angeles |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2017 |
| Subject Keyword | cuprizone animal model remyelination oral administration demyelination |
| Content Type | Text |
| Resource Type | Article |
| Subject | Neuroscience Neurology (clinical) |
