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Role of collectin-11 in innate defence against uropathogenic Escherichia coli infection
| Content Provider | SAGE Publishing |
|---|---|
| Author | Yuan, Hai Gao, Zhao Lu, Xiaohan Hu, Fengqi |
| Copyright Year | 2020 |
| Abstract | Classical collectins (surfactant protein A and D) play a significant role in innate immunity and host defence in uropathogenic Escherichia coli (UPEC)-induced urinary tract infection (UTI). However, the functions of collectin-11 (CL-11) with respect to UPEC and UTI remain largely unexplored. This study aimed to investigate the effect of CL-11 on UPEC and its role in UTI. We further examined its modulatory effect on inflammatory reactions in proximal tubular epithelial cells (PTECs). The present study provides evidence for the effect of CL-11 on the growth, agglutination, binding, epithelial adhesion and invasion of UPEC. We found increased basal levels of phosphorylated p38 MAPK and human cytokine homologue (keratinocyte-derived chemokine) expression in CL-11 knockdown PTECs. Furthermore, signal regulatory protein α blockade reversed the increased basal levels of inflammation associated with CL-11 knockdown in PTECs. Additionally, CL-11 knockdown effectively inhibited UPEC-induced p38 MAPK phosphorylation and cytokine production in PTECs. These were further inhibited by CD91 blockade. We conclude that CL-11 functions as a mediator of innate immunity via direct antibacterial roles as well as dual modulatory roles in UPEC-induced inflammatory responses during UTI. Thus, the study findings suggest a possible function for CL-11 in defence against UTI. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/1753425920974766?download=true |
| Starting Page | 50 |
| Ending Page | 60 |
| Page Count | 11 |
| ISSN | 17534259 |
| Issue Number | 1 |
| Volume Number | 27 |
| Journal | Innate Immunity (INI) |
| e-ISSN | 17534267 |
| DOI | 10.1177/1753425920974766 |
| Language | English |
| Publisher | Sage Publications UK |
| Publisher Date | 2020-11-26 |
| Publisher Place | London |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2020 |
| Subject Keyword | Collectin-11 innate immunity urinary tract infection uropathogenic Escherichia coli |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Cell Biology Molecular Biology Immunology Microbiology |
