Loading...
Please wait, while we are loading the content...
Similar Documents
Clinical Value of Serum and Exhaled Breath Condensate miR-186 and IL-1β Levels in Non-Small Cell Lung Cancer
| Content Provider | SAGE Publishing |
|---|---|
| Author | Xie, Haiqin Chen, Jinliang Lv, Xuedong Zhang, Lu Wu, Jinnan Ge, Xin Yang, Qichang Zhang, Dongmei Chen, Jianrong |
| Copyright Year | 2020 |
| Abstract | Objective:Our study aimed to investigate the expression level and clinical significance of serum and exhaled breath condensate miR-186 and IL-1β in non-small cell lung cancer patients.Methods:The serum and exhaled breath condensate specimens of 62 non-small cell lung cancer patients and 60 healthy controls were collected to detect miR-186 expression levels by real-time fluorescent quantitative PCR. Enzyme linked immunosorbent assay was applied to examine IL-1β concentration. Statistical analyses were used to evaluate the correlation between miR-186 and IL-1β in serum and clinicopathological features, traditional serum tumor markers, and inflammatory markers. The diagnostic efficacy of miR-186 and IL-1β for non-small cell lung cancer was evaluated by receiver operating characteristic curve analysis. The correlation between miR-186 and IL-1β was determined.Results:① The relative expression level of miR-186 was greatly reduced in the serum and EBC of patients with non-small cell lung cancer, and the miR-186 expression level was reduced in different TNM stages of non-small cell lung cancer, from the early to later stages. ② The IL-1β concentration in serum and exhaled breath condensate of patients with non-small cell lung cancer was increased. ③ Serum miR-186 and IL-1β levels were closely related to lymph node metastasis, and the low expression of serum miR-186 and the high concentration of IL-1β were associated with higher serum carcinoembryonic antigen, C-reactive protein, and erythrocyte sedimentation rate levels. ④ ROC curve analysis showed that exhaled breath condensate miR-186 had higher area under the curve than serum miR-186, and the combined detection showed higher diagnostic efficacy than the separate detection. In addition, the combined detection of IL-1β and miR-186 has a larger AUC than the separate detection of both. ⑤ The correlation between serum miR-186 and IL-1β was negative.Conclusion:miR-186 and IL-1β are expected to be potential diagnostic biomarkers for non-small cell lung cancer. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/1533033820947490?download=true |
| ISSN | 15330346 |
| Volume Number | 19 |
| Journal | Technology in Cancer Research & Treatment (TCT) |
| e-ISSN | 15330338 |
| DOI | 10.1177/1533033820947490 |
| Language | English |
| Publisher | Sage Publications CA |
| Publisher Date | 2020-08-27 |
| Publisher Place | Los Angeles |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2020 |
| Subject Keyword | interleukin-1β exhaled breath condensate non-small cell lung cancer miR-186 diagnosis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cancer Research Oncology |
