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MicroRNA-325 Is a Potential Biomarker and Tumor Regulator in Human Bladder Cancer
| Content Provider | SAGE Publishing |
|---|---|
| Author | Lin, Tao Zhou, Shiming Gao, Hui Li, Yuqiao Sun, Lijiang |
| Copyright Year | 2018 |
| Abstract | We evaluated whether human microRNA-325 may be a potential biomarker and tumor regulator in bladder cancer.Methods:Human microRNA-325 expression was probed by quantitative real-time polymerase chain reaction in both in vitro bladder cancer cell lines and in vivo bladder carcinoma tissues retrieved from patients with cancer. The prognostic potential of human microRNA-325 in predicting postoperative overall survival of patients with bladder cancer was estimated. Endogenous human microRNA-325 was overexpressed by lentiviral transduction in bladder cancer cell lines, T24 and 5637 cells. The tumor regulatory effects of human microRNA-325 upregulation on T24 and 5637 cells were evaluated both in vitro and in vivo.Results:Human microRNA-325 was aberrantly downregulated in both bladder cell lines and human bladder carcinomas. Lowly expressed human microRNA-325 in bladder carcinoma was closely associated with poor postoperative overall survival of patients with cancer. In T24 and 5637 cells, virally transduced cells had markedly upregulated human microRNA-325 expressions. Biochemical assays demonstrated that human microRNA-325 upregulation in bladder cancer had tumor-suppressive functions by decreasing cancer proliferation, cisplatin chemoresistance, and cancer migration in vitro and hindering transplantation growth in vivo and cell cycle transition.Conclusion:Human microRNA-325 is lowly expressed and may serve as a potential prognostic biomarker in human bladder cancer. After further validation, human microRNA-325 may be a novel therapeutic target for suppressing carcinoma in patients with bladder cancer. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/1533033818790536?download=true |
| ISSN | 15330346 |
| Volume Number | 17 |
| Journal | Technology in Cancer Research & Treatment (TCT) |
| e-ISSN | 15330338 |
| DOI | 10.1177/1533033818790536 |
| Language | English |
| Publisher | Sage Publications CA |
| Publisher Date | 2018-09-03 |
| Publisher Place | Los Angeles |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2018 |
| Subject Keyword | cisplatin overall survival miR-325 proliferation bladder cancer prognosis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cancer Research Oncology |
