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Detection of Anti-SARS-CoV-2 Nucleocapsid and Spike Antibodies in Patients with Coronavirus Disease 2019 in Japan
| Content Provider | SAGE Publishing |
|---|---|
| Author | Furukawa, Hiroshi Oka, Shomi Higuchi, Takashi Yamaguchi, Miho Uchiyama, Shota Koiwa, Tomohiro Nakama, Moriyuki Minegishi, Masaaki Nagai, Hideaki Tohma, Shigeto |
| Copyright Year | 2022 |
| Abstract | OBJECTIVESCoronavirus Disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Serological testing for anti-SARS-CoV-2 nucleocapsid (N) antibodies (Abs) and anti-SARS-CoV-2 spike (S) Abs is performed to detect prior COVID-19 infection. It is still controversial which antibodies are the most sensitive and specific, and which can be detected earliest after infection. Here, we evaluated the results of serological tests of anti-SARS-CoV-2 N and S Abs in Japan.METHODSSymptomatic COVID-19 patients (n = 84) and control patients with rheumatoid arthritis (n = 93) were recruited at Tokyo National Hospital. Anti-SARS-CoV-2 N and S Abs were measured by commercial electrochemiluminescence immunoassays.RESULTSThe fraction of patients positive for anti-SARS-CoV-2 N and S Abs was highest >14 days after symptom onset. The frequency of anti-SARS-CoV-2 S Ab positivity at this time (80.4%) tended to be slightly but not significantly lower than anti-SARS-CoV-2 N Ab positivity (84.8%). Optimized cut-off levels for anti-SARS-CoV-2 N and S Ab positivity were lower than the manufacturer's recommended cut-off levels. Using multiple linear regression analyzes with anti-SARS-CoV-2 N and S Abs, we created an Ab-index with high sensitivity.CONCLUSIONTo increase the sensitivity of serological diagnostic tests for COVID-19, it is suggested that both anti-SARS-CoV-2 N and S Abs should be measured and cut-off levels decreased. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/11795484221075492?download=true |
| ISSN | 29768675 |
| Volume Number | 16 |
| Journal | Clinical Medicine Insights: Circulatory, Respiratory and Pulmonary Medicine (CRA) |
| e-ISSN | 29768675 |
| DOI | 10.1177/11795484221075492 |
| Language | English |
| Publisher | Sage Publications UK |
| Publisher Date | 2022-04-05 |
| Publisher Place | London |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2022 |
| Subject Keyword | COVID-19 anti-SARS-CoV-2 antibody nucleocapsid electrochemiluminescence immunoassay spike |
| Content Type | Text |
| Resource Type | Article |
