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In Situ-Formed Fibrin Hydrogel Scaffold Loaded With Human Umbilical Cord Mesenchymal Stem Cells Promotes Skin Wound Healing
| Content Provider | SAGE Publishing |
|---|---|
| Author | Hu, Lvzhong Zhou, Jinhua He, Zhisong Zhang, Lin Du, Fangzhou Nie, Mengting Zhou, Yao Hao, Hang Zhang, Lixing Yu, Shuang Zhang, Jingzhong Chen, Youguo |
| Copyright Year | 2023 |
| Abstract | Healing of full-thickness skin wounds remains a major challenge. Recently, human umbilical cord mesenchymal stem cells (hUC-MSCs) were shown to possess an extraordinary potential to promote skin repair in clinical settings. However, their low survival rate after transplantation limits their therapeutic efficiency in treating full-thickness skin wounds. Hydrogels are considered an ideal cell transplantation vector owing to their three-dimensional mesh structure, good biosafety, and biodegradation. The objective of this study was to investigate the skin wound healing effect of a fibrin hydrogel scaffold loaded with hUC-MSCs. We found that the fibrin hydrogel had a three-dimensional mesh structure and low cytotoxicity and could prolong the time of cell survival in the peri-wound area. The number of green fluorescent protein (GFP)-labeled hUC-MSCs was higher in the full-thickness skin wound of mice treated with hydrogel–hUC-MSCs than those of mice treated with cell monotherapy. In addition, the combination therapy between the hydrogel and hUC-MSCs speed up wound closure, its wound healing rate was significantly higher than those of phosphate-buffered saline (PBS) therapy, hydrogel monotherapy, and hUC-MSCs monotherapy. Furthermore, the results showed that the combination therapy between hydrogel and hUC-MSCs increased keratin 10 and keratin 14 immunofluorescence staining, and upregulated the relative gene expressions of epidermal growth factor (EGF), transforming growth factor-β1 (TGF-β1), and vascular endothelial growth factor A (VEGFA), promoting epithelial regeneration and angiogenesis. In conclusion, the fibrin hydrogel scaffold provides a relatively stable sterile environment for cell adhesion, proliferation, and migration, and prolongs cell survival at the wound site. The hydrogel–hUC-MSCs combination therapy promotes wound closure, re-epithelialization, and neovascularization. It exhibits a remarkable therapeutic effect, being more effective than the monotherapy with hUC-MSCs or hydrogel. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/09636897231156215?download=true |
| ISSN | 09636897 |
| Volume Number | 32 |
| Journal | Cell Transplantation (CLL) |
| e-ISSN | 15553892 |
| DOI | 10.1177/09636897231156215 |
| Language | English |
| Publisher | Sage Publications CA |
| Publisher Date | 2023-02-25 |
| Publisher Place | Los Angeles |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2023 |
| Subject Keyword | skin wound healing fibrin human umbilical cord mesenchymal stem cells hydrogel |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Transplantation Biomedical Engineering |
