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Neurotrophic Factor Secretion and Neural Differentiation Potential of Multilineage-differentiating Stress-enduring (Muse) Cells Derived from Mouse Adipose Tissue
| Content Provider | SAGE Publishing |
|---|---|
| Author | Nitobe, Yohshiro Nagaoki, Toshihide Kumagai, Gentaro Sasaki, Ayako Liu, Xizhe Fujita, Taku Fukutoku, Tatsuhiro Wada, Kanichiro Tanaka, Toshihiro Kudo, Hitoshi Asari, Toru Furukawa, Ken-Ichi Ishibashi, Yasuyuki |
| Copyright Year | 2019 |
| Abstract | Multilineage-differentiating stress-enduring (Muse) cells are endogenous pluripotent stem cells that can be isolated based on stage-specific embryonic antigen-3 (SSEA-3), a pluripotent stem cell-surface marker. However, their capacities for survival, neurotrophic factor secretion, and neuronal and glial differentiation are unclear in rodents. Here we analyzed mouse adipose tissue-derived Muse cells in vitro. We collected mesenchymal stem cells (MSCs) from C57BL/6 J mouse adipose tissue and separated SSEA-3+, namely Muse cells, and SSEA-3–, non-Muse cells, to assess self-renewability; pluripotency marker expression (Nanog, Oct3/4, Sox2, and SSEA-3); spontaneous differentiation into endodermal, mesodermal, and ectodermal lineages; and neural differentiation capabilities under cytokine induction. Neurally differentiated Muse and non-Muse cell functions were assessed by calcium imaging. Antioxidant ability was measured to assess survival under oxidative stress. Brain-derived neurotrophic factor (BDNF), vascular endothelial cell growth factor (VEGF), and hepatocyte growth factor (HGF) secretion were analyzed in enzyme-linked immunosorbent assays. SSEA-3+ Muse cells (6.3 ± 1.9% of mouse adipose-MSCs), but not non-Muse cells, exhibited self-renewability, spontaneous differentiation into the three germ layers, and differentiation into cells positive for Tuj-1 (27 ± 0.9%), O4 (17 ± 3.4%), or GFAP (23 ± 1.3%) under cytokine induction. Neurally differentiated Muse cells responded to KCl depolarization with greater increases in cytoplasmic Ca2+ levels than non-Muse cells. Cell survival under oxidative stress was significantly higher in Muse cells (50 ± 2.7%) versus non-Muse cells (22 ± 2.8%). Muse cells secreted significantly more BDNF, VEGF, and HGF (273 ± 12, 1479 ± 7.5, and 6591 ± 1216 pg/mL, respectively) than non-Muse cells (133 ± 4.0, 1165 ± 20, and 2383 ± 540 pg/mL, respectively). Mouse Muse cells were isolated and characterized for the first time. Muse cells showed greater pluripotency-like characteristics, survival, neurotrophic factor secretion, and neuronal and glial-differentiation capacities than non-Muse cells, indicating that they may have better neural-regeneration potential. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/0963689719863809?download=true |
| Starting Page | 1132 |
| Ending Page | 1139 |
| Page Count | 8 |
| ISSN | 09636897 |
| Issue Number | 9-10 |
| Volume Number | 28 |
| Journal | Cell Transplantation (CLL) |
| e-ISSN | 15553892 |
| DOI | 10.1177/0963689719863809 |
| Language | English |
| Publisher | Sage Publications CA |
| Publisher Date | 2019-07-15 |
| Publisher Place | Los Angeles |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2019 |
| Subject Keyword | mouse adipose tissue neurotrophic factor muse cell neuroregeneration |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Transplantation Biomedical Engineering |
