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HSP60 activity on human bronchial epithelial cells

Content Provider	SAGE Publishing
Author	Sangiorgi, Claudia Vallese, Davide Gnemmi, Isabella Bucchieri, Fabio Balbi, Bruno Brun, Paola Leone, Angelo Giordano, Andrea Everly Conway de Macario Macario, Alberto Jl Cappello, Francesco Stefano, Antonino Di
Copyright Year	2017
Abstract	HSP60 has been implicated in chronic inflammatory disease pathogenesis, including chronic obstructive pulmonary disease (COPD), but the mechanisms by which this chaperonin would act are poorly understood. A number of studies suggest a role for extracellular HSP60, since it can be secreted from cells and bind Toll-like receptors; however, the effects of this stimulation have never been extensively studied. We investigated the effects (pro- or anti-inflammatory) of HSP60 in human bronchial epithelial cells (16-HBE) alone and in comparison with oxidative, inflammatory, or bacterial challenges. 16-HBE cells were cultured for 1–4 h in the absence or presence of HSP60, H2O2, lipopolysaccharide (LPS), or cytomix. The cell response was evaluated by measuring the expression of IL-8 and IL-10, respectively, pro- and anti-inflammatory cytokines involved in COPD pathogenesis, as well as of pertinent TLR-4 pathway mediators. Stimulation with HSP60 up-regulated IL-8 at mRNA and protein levels and down-regulated IL-10 mRNA and protein. Likewise, CREB1 mRNA was up-regulated. H2O2 and LPS up-regulated IL-8. Experiments with an inhibitor for p38 showed that this mitogen-activated protein kinase could be involved in the HSP60-mediated pro-inflammatory effects. HSP60 showed pro-inflammatory properties in bronchial epithelial cells mediated by activation of TLR-4-related molecules. The results should prompt further studies on more complex ex-vivo or in-vivo models with the aim to elucidate further the role of those molecules in the pathogenesis of COPD.
Related Links	https://journals.sagepub.com/doi/pdf/10.1177/0394632017734479?download=true
Starting Page	333
Ending Page	340
Page Count	8
ISSN	03946320
Issue Number	4
Volume Number	30
Journal	International Journal of Immunopathology and Pharmacology (IJI)
e-ISSN	20587384
DOI	10.1177/0394632017734479
Language	English
Publisher	Sage Publications UK
Publisher Date	2017-10-04
Publisher Place	London
Access Restriction	Open
Rights Holder	© The Author(s) 2017
Subject Keyword	ERK1 16-HBE CREB1 HSP60 MyD88 IL-10 p38α IL-8 NF-κB p65 subunit COPD JNK1 TLR-4
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Pharmacology Immunology

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