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Effects of naringin on reversing cisplatin resistance and the Wnt/β-catenin pathway in human ovarian cancer SKOV3/CDDP cells
| Content Provider | SAGE Publishing |
|---|---|
| Author | Zhu, Hong Zou, Xia Lin, ShiXin Hu, Xin Gao, Jun |
| Copyright Year | 2019 |
| Abstract | ObjectiveOvarian cancer is one of three malignant tumors of the female reproductive system. Our previous studies showed that the traditional Chinese medicine naringin significantly inhibited the proliferation of platinum-resistant ovarian cancer cells in vitro, and that the mechanism may be related to the NF-κB pathway.MethodsThe MTT assay was used to detect the sensitivity of SKOV3 and SKOV3/CDDP cells to cisplatin, the effect of different naringin concentrations on the proliferation of SKOV3/CDDP cells, and the reversal of cisplatin resistance in naringin-treated SKOV3/CDDP cells. Western blotting was used to detect β-catenin, c-Myc, and cyclin D1 protein levels in the different cell lines.ResultsMTT results showed that different concentrations of naringin inhibited the proliferation of SKOV3 and SKOV3/CDDP cells, and that the inhibition increased with increasing concentrations and prolonged incubation times. Western blotting revealed that compared with controls (SKOV3/CDDP-0), β-catenin, c-Myc and cyclin D1 proteins levels were significantly decreased in SKOV3/CDDP-C, SKOV3/CDDP-N 20, and SKOV3/CDDP-CN 20 cells, suggesting that naringin inhibited the proliferation of SKOV3/CDDP cells in a concentration and time dependent manner.ConclusionsNon-cytotoxic naringin reduced the expression of β-catenin, c-Myc, and cyclin D1 in SKOV3/CDDP cells and partially reversed cisplatin resistance in SKOV3/CDDP CN 20 cells. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/0300060519887869?download=true |
| ISSN | 03000605 |
| Issue Number | 10 |
| Volume Number | 48 |
| Journal | Journal of International Medical Research (IMR) |
| e-ISSN | 14732300 |
| DOI | 10.1177/0300060519887869 |
| Language | English |
| Publisher | Sage Publications UK |
| Publisher Date | 2020-10-22 |
| Publisher Place | London |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2019 |
| Subject Keyword | cisplatin SKOV3/CDDP Wnt/β-catenin signaling pathway cisplatin resistance Ovarian cancer naringin |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry (medical) Cell Biology Biochemistry |
