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Validating Bioluminescence Imaging as a High-Throughput, Quantitative Modality for Assessing Tumor Burden
| Content Provider | SAGE Publishing |
|---|---|
| Author | Paroo, Zain Bollinger, Robert A. Braasch, Dwaine A. Richer, Edmond Corey, David R. Antich, Peter P. Mason, Ralph P. |
| Copyright Year | 2004 |
| Abstract | Bioluminescence imaging (BLI) is a highly sensitive tool for visualizing tumors, neoplastic development, metastatic spread, and response to therapy. Although BLI has engendered much excitement due to its apparent simplicity and ease of implementation, few rigorous studies have been presented to validate the measurements. Here, we characterize the nature of bioluminescence output from mice bearing subcutaneous luciferase-expressing tumors over a 4-week period. Following intraperitoneal or direct intratumoral administration of luciferin substrate, there was a highly dynamic kinetic profile of light emission. Although bioluminescence was subject to variability, strong correlations (r > .8, p < .001) between caliper measured tumor volumes and peak light signal, area under light signal curve and light emission at specific time points were determined. Moreover, the profile of tumor growth, as monitored with bioluminescence, closely resembled that for caliper measurements. The study shows that despite the dynamic and variable nature of bioluminescence, where appropriate experimental precautions are taken, single time point BLI may be useful for noninvasive, high-throughput, quantitative assessment of tumor burden. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1162/15353500200403172?download=true |
| ISSN | 15353508 |
| Issue Number | 2 |
| Volume Number | 3 |
| Journal | Molecular Imaging (MIX) |
| e-ISSN | 15360121 |
| DOI | 10.1162/15353500200403172 |
| Language | English |
| Publisher | Sage Publications CA |
| Publisher Date | 2004-04-01 |
| Publisher Place | Los Angeles |
| Access Restriction | Open |
| Rights Holder | © 2004 Massachusetts Institute of Technology |
| Subject Keyword | luciferin noninvasive imaging HeLa cells Luciferase bioluminescent imaging |
| Content Type | Text |
| Resource Type | Article |
| Subject | Radiology, Nuclear Medicine and Imaging Molecular Medicine Condensed Matter Physics Biomedical Engineering Biotechnology |
