NDLI: Epigenetic toxicity of trichloroethylene: a single-molecule perspective

Please wait, while we are loading the content...

Beneficial protective effects of 2-allyl amino 4-methyl sulfanyl butyric acid on glucose metabolism and glycoprotein components in streptozotocin induced diabetic rats with molecular modeling

Health hazards of methylammonium lead iodide based perovskites: cytotoxicity studies

Testicular and epididymal toxicity induced by benzo(a)pyrene, alcohol, and their combination in Wistar rats

Alkyl chain modulated cytotoxicity and antioxidant activity of bioinspired amphiphilic selenolanes

Genotoxic risk of quinocetone and its possible mechanism in in vitro studies

Involvement of ROS-mediated mitochondrial dysfunction and SIRT3 down-regulation in tris(2-chloroethyl)phosphate-induced cell cycle arrest

Tri-m-cresyl phosphate and PPAR/LXR interactions in seabream hepatocytes: revealed by computational modeling (docking) and transcriptional regulation of signaling pathways

Physicochemical insights of irradiation-enhanced hydroxyl radical generation from ZnO nanoparticles

Multi-walled carbon nanotubes enhanced fungal colonization and suppressed innate immune response to fungal infection in nematodes

Accumulation, biotransformation, and multi-biomarker responses after exposure to arsenic species in the earthworm Eisenia fetida

A possible new mechanism and drug intervention for kidney damage due to arsenic poisoning in rats

Mycotoxin ochratoxin A disrupts renal development via a miR-731/prolactin receptor axis in zebrafish

The urinary excretion of an organic anion transporter as an early biomarker of methotrexate-induced kidney injury

Effects of perfluorooctane sulfonate and its alternatives on long-term potentiation in the hippocampus CA1 region of adult rats in vivo

Low-dose chemotherapeutic drugs induce reactive oxygen species and initiate apoptosis-mediated genomic instability

High-dose consumption of NaCl resulted in severe degradation of lipoproteins associated with hyperlipidemia, hyperglycemia, and infertility via impairment of testicular spermatogenesis

In silico prediction of hERG potassium channel blockage by chemical category approaches

Dynamic cytotoxic profiles of sulfur mustard in human dermal cells determined by multiparametric high-content analysis

Dephosphorylation of Tak1 at Ser412 greatly contributes to the spermatocyte-specific testis toxicity induced by (5R)-5-hydroxytriptolide in C57BL/6 mice

Silver nanoparticles induced oxidative and endoplasmic reticulum stresses in mouse tissues: implications for the development of acute toxicity after intravenous administration

Genome-wide transcriptional analysis of silica nanoparticle-induced toxicity in zebrafish embryos

Gene expression profiling to identify the toxicities and potentially relevant disease outcomes due to endosulfan exposure

Trichloroethylene-induced hypersensitivity dermatitis was associated with hepatic metabolic enzyme genes and immune-related genes

Epigenetic toxicity of trichloroethylene: a single-molecule perspective

Differential expression of serum proteins in rats subchronically exposed to arsenic identified by iTRAQ-based proteomic technology—14-3-3 ζ protein to serve as a potential biomarker

Uranium-induced rat kidney cell cytotoxicity is mediated by decreased endogenous hydrogen sulfide (H₂S) generation involved in reduced Nrf2 levels

Long-term exposure to diesel engine exhaust affects cytokine expression among occupational population

Arsenic trioxide triggered calcium homeostasis imbalance and induced endoplasmic reticulum stress-mediated apoptosis in adult rat ventricular myocytes

¹H NMR-based urine metabolomics for the evaluation of kidney injury in Wistar rats by 3-MCPD

In vitro toxicological evaluation of ethyl carbamate in human HepG2 cells

The binary, ternary and quaternary mixture toxicity of benzo[a]pyrene, arsenic, cadmium and lead in HepG2 cells

Comment on “Promising blood-derived biomarkers for estimation of the postmortem interval” by I. Costa, F. Carvalho, T. Magalhães, P. G. de Pinho, R. Silvestre & R. J. Dinis-Oliveira. (Toxicol. Res., 2015, 4, 1443–1452)

Response to the comment on “Promising blood-derived biomarkers for estimation of the postmortem interval”

Back cover

Toxicology Research Vol - 5 Issue - 1

Toxicology Research Vol -4

Toxicology Research Vol -3

Toxicology Research Vol -2

Toxicology Research Vol -1

Epigenetic toxicity of trichloroethylene: a single-molecule perspective

Content Provider	Royal Society of Chemistry (RSC)
Author	Irudayaraj, Joseph Roy Choudhury, Samrat Cui, Yi
Copyright Year	2016
Abstract	The volatile, water soluble trichloroethylene (TCE) is a hazardous industrial waste and could lead to various health problems, including cancer, neuropathy, cardiovascular defects, and immune diseases. Toxicological studies using in vitro and in vivo models have been conducted to understand the biological impacts of TCE at the genetic, transcriptomic, metabolomic, and signaling levels. The epigenetic aberrations induced by TCE have also been reported in a number of model organisms, while a detailed mechanistic elucidation is lacking. In this study we uncover an unreported mechanism accounting for the epigenetic toxicity due to TCE exposure by monitoring the single-molecule dynamics of DNA methyltransferase 3a (Dnmt3a) in living cells. TCE-induced global DNA hypomethylation could be partly attributed to the disrupted Dnmt3a–DNA association. By analyzing the components of detached Dnmt3a, we found that the Dnmt3a oligomers (e.g., dimer, trimer, and high-order oligomers) dissociated from heterochromatin in a dose-dependent manner upon exposure. Thereafter the diminished DNA-binding affinity of Dnmt3a resulted in a significant decrease in 5-methylcytosine (5mC) under both acute high-dosage and chronic low-dosage TCE exposure. The resulting DNA demethylation might also be contributed by the elevated expression of ten-eleven-translocation (Tet) enzymes and a reformed cysteine cycle. Besides the global effect, we further identified that a group of heterochromatin-located, cancer-related microRNAs (miRNAs) experienced promoter demethylation upon TCE exposure.
Starting Page	641
Ending Page	650
Page Count	10
File Format	HTM / HTML PDF
ISSN	2045452X
Volume Number	5
Issue Number	2
Journal	Toxicology Research
DOI	10.1039/c5tx00454c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Metabolomics Protein trimer Cysteine 5-Methylcytosine Trichloroethylene DNA demethylation Peripheral neuropathy Protein dimer Heterochromatin DNA TCE Epigenetics DNA methyltransferase Cancer
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Toxicology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Epigenetic Toxicity of Trichloroethylene: A Single-Molecule Perspective.

DNA 5-Methylcytosine Demethylation Activities of the Mammalian DNA Methyltransferases

Epigenetic inactivation of the 5-methylcytosine RNA methyltransferase NSUN7 is associated with clinical outcome and therapeutic vulnerability in liver cancer

Targeted DNA demethylation in human cells by fusion of a plant 5-methylcytosine DNA glycosylase to a sequence-specific DNA binding domain

Demethylation of Non-CpG Sites in DNA Is Initiated by TET2 5-Methylcytosine Dioxygenase

Transient reduction of 5-methylcytosine and 5-hydroxymethylcytosine is associated with active DNA demethylation during regeneration of zebrafish fin

Epigenetic DNA-(cytosine-5-carbon) modifications: 5-aza-2′-deoxycytidine and DNA-demethylation

Identification of epigenetic DNA modifications with a protein nanopore.

Global DNA demethylation as an epigenetic marker of human brain metastases.

Epigenetic toxicity of trichloroethylene: a single-molecule perspective

Similar Documents

Epigenetic Toxicity of Trichloroethylene: A Single-Molecule Perspective.

DNA 5-Methylcytosine Demethylation Activities of the Mammalian DNA Methyltransferases

Epigenetic inactivation of the 5-methylcytosine RNA methyltransferase NSUN7 is associated with clinical outcome and therapeutic vulnerability in liver cancer

Targeted DNA demethylation in human cells by fusion of a plant 5-methylcytosine DNA glycosylase to a sequence-specific DNA binding domain

Demethylation of Non-CpG Sites in DNA Is Initiated by TET2 5-Methylcytosine Dioxygenase

Transient reduction of 5-methylcytosine and 5-hydroxymethylcytosine is associated with active DNA demethylation during regeneration of zebrafish fin

Epigenetic DNA-(cytosine-5-carbon) modifications: 5-aza-2′-deoxycytidine and DNA-demethylation

Identification of epigenetic DNA modifications with a protein nanopore.

Global DNA demethylation as an epigenetic marker of human brain metastases.

Epigenetic toxicity of trichloroethylene: a single-molecule perspective