NDLI: Development of a single-step immunoassay microdevice based on a graphene oxide-containing hydrogel possessing fluorescence quenching and size separation functions

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The Analyst Vol - 142 Issue - 2

Development of a single-step immunoassay microdevice based on a graphene oxide-containing hydrogel possessing fluorescence quenching and size separation functions

Content Provider	Royal Society of Chemistry (RSC)
Author	Sueyoshi, Kenji Nakashima, Kaho Endo, Tatsuro Shirai, Akihiro Hisamoto, Hideaki
Copyright Year	2017
Abstract	An immunoassay, which is an indispensable analytical method both in biological research and in medical fields was successfully integrated into a “single-step” by developing a microdevice composed of a graphene oxide (GO)-containing hydrogel and a poly (dimethylsiloxane) (PDMS) microchannel array with a polyethylene glycol (PEG) coating containing a fluorescently-labelled antibody. Here we used 2-hydroxyethylmethacrylate (HEMA) as a monomer that is easily, and homogeneously, mixed with GO to synthesize the hydrogel. The fluorescence quenching and size separation functions were then optimized by controlling the ratios of HEMA and GO. Free fluorescently-labelled antibody was successfully separated from the immunoreaction mixture by the hydrogel network structure, and the fluorescence was subsequently quenched by GO. In comparison to the previously reported immunoassay system using GO, the present system achieved a very high fluorescence resonance energy transfer (FRET) efficiency (∼90%), due to the use of direct adsorption of the fluorescently-labelled antibody to the GO surface; in contrast, the former reported method relied on indirect adsorption of the fluorescently-labelled antibody via immunocomplex formation at the GO surface. Finally, the single-step immunoassay microdevice was made by combining the developed hydrogel and the PDMS microchannel with a coating containing the fluorescently-labelled antibody, and successfully applied for the single-step analysis of IgM levels in diluted human serum by simple introduction of the sample via capillary action.
Starting Page	472
Ending Page	477
Page Count	6
File Format	HTM / HTML PDF
ISSN	00032654
Volume Number	142
Issue Number	3
Journal	The Analyst
DOI	10.1039/c6an02485h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Immunoglobulin M Capillary action Immunoassay Förster resonance energy transfer Historical European martial arts Adsorption Graphene Polyethylene glycol (Hydroxyethyl)methacrylate Fluorescence Monomer Gel
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry Electrochemistry

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