NDLI: Changes in molecular composition and packing during lipid membrane reconstitution from phospholipid

Content Provider	Royal Society of Chemistry (RSC)
Author	Lee, Chongsoo Bain, Colin D. Wacklin, Hanna
Copyright Year	2009
Abstract	Total internal reflection (TIR) Raman spectroscopy was used to investigate changes in the properties of phospholipid bilayers at the quartz–water interface during the process of membrane reconstitution from lipid–detergent micelles. The non-ionic detergent β-D-dodecyl maltoside (β-DDM) was used in 9 : 1 ratio to deuterated and non-deuterated dipalmitoylphosphatidyl choline (DPPC and d75-DPPC) to form detergent–lipid micelles. Exposure of the quartz surface to decreasing concentrations of such detergent–lipid solutions resulted in the formation of a d75-DPPC bilayer indistinguishable from that of a bilayer formed by fusion of small unilamellar vesicles of the pure lipid, indicating that the use of detergent micelles does not alter the packing or conformation of lipids, or leave a residue of surfactant in the bilayer. Polarised measurements of d75DPPC spectra in the C–H and C–D regions at each step of the process showed that the lipid surface density increases as the detergent is removed by rinsing and replaced by more lipid. The absence of shifts in the frequencies demonstrates that DPPC is in a gel-like conformation even at high relative surfactant fractions. The TIR geometry also allowed us to record high resolution spectra in the low frequency C–D bending region of d75-DPPC, which showed that the lipid molecules have an upright orientation with respect to the support surface.
Starting Page	568
Ending Page	575
Page Count	8
File Format	HTM / HTML PDF
ISSN	1744683X
Volume Number	5
Issue Number	3
Journal	Soft Matter
DOI	10.1039/b812768a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Detergent Support surface Phospholipid Raman spectroscopy Quartz Surfactant Lipid Molecular geometry Total internal reflection Choline Raman Protein structure DPPC Dipalmitoylphosphatidylcholine
Content Type	Text
Resource Type	Article
Subject	Chemistry Condensed Matter Physics

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