NDLI: Polymer-tethered membranes as quantitative models for the study of integrin-mediated cell adhesion

Content Provider	Royal Society of Chemistry (RSC)
Author	Jordan, Rainer Bärmann, Michael Purrucker, Oliver Tanaka, Motomu Gönnenwein, Stefanie Förtig, Anton Sackmann, Erich Rusp, Monika Moroder, Luis
Copyright Year	2007
Abstract	Here we report a remarkable enhancement in the adhesion strength of transmembrane cell receptors, human platelet integrin, in a new class of supported lipid membranes, which are separated from the solid substrates by linear polymer spacers. The amphiphilic polymer tether consists of linear hydrophilic poly(2-oxazoline) chains of defined length (degree of polymerizationn = 104, MW/Mn = 1.30), whose chain termini are functionalized with the tri-functional silane surface coupling group and hydrophobic n-alkyl chains as membrane anchors (lipopolymers). As a model of test cells, giant lipid vesicles were functionalized with synthetic ligand molecules containing the RGD sequence, and the free energy of adhesion Δgad between the integrin-doped tethered membrane and the vesicle was measured using a micro-interferometry technique. It has been demonstrated that the adhesion function of integrin receptors in these polymer-tethered membranes is 30 times stronger than those incorporated into membranes directly deposited onto solid substrates (solid-supported membranes). The obtained results demonstrate that linear lipopolymer spacers provide a fluid and non-denaturing environment for the incorporated cell receptors and allow quantitative modelling of cell adhesion processes.
Starting Page	333
Ending Page	336
Page Count	4
File Format	HTM / HTML PDF
ISSN	1744683X
Volume Number	3
Issue Number	3
Journal	Soft Matter
DOI	10.1039/b612069e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Platelet Integrin Oxazoline Ligand Cell adhesion Polymer Amphiphile Hydrophile Hydrophobe Lipid Silane Thermodynamic free energy
Content Type	Text
Resource Type	Article
Subject	Chemistry Condensed Matter Physics

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