NDLI: Sequential crosslinking to control cellular spreading in 3-dimensional hydrogels

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Soft Matter Vol - 5 Issue - 7

Soft Matter Vol - 5 Issue - 6

Soft Matter Vol - 5 Issue - 5

Soft Matter Vol - 5 Issue - 4

Soft Matter Vol - 5 Issue - 3

Sequential crosslinking to control cellular spreading in 3-dimensional hydrogels

Content Provider	Royal Society of Chemistry (RSC)
Author	Burdick, Jason A. Katz, Joshua S. Khetan, Sudhir
Copyright Year	2009
Abstract	With advanced understanding of how manipulations in material chemistry and structure influence cellular interactions, material control over cellular behavior (e.g., spreading) is becoming increasingly possible. In this example, we developed a novel process that utilizes different crosslinking mechanisms to provide gel environments that are either permissive or inhibitory to cellular spreading. To accomplish this, a multi-acrylated macromer (i.e., acrylated hyaluronic acid) was first crosslinked with an addition reaction using a matrix metalloprotease (MMP) cleavable peptide containing thiol groups. When an adhesive peptide was also coupled to the network, this environment permitted the spreading of encapsulated human mesenchymal stem cells (hMSCs), whereas control systems did not. If all acrylates were not consumed during the initial crosslinking step, a photoinitiated radical polymerization could be used to crosslink the remaining acrylates and inhibit cellular spreading with the production of covalent barriers. Variations in the ratio of the two crosslink types in individual constructs controlled the degradation and mechanical properties of the hydrogels, as well as the degree of spreading of encapsulated cells. Cell spreading was further controlled spatially with the use of photomasks. Overall, this new technology is an exciting and potentially valuable tool, both to provide new insights into the relationships between gel structure and cell behavior, and for eventual tissue-engineering applications where spatial control over cells is desired.
Starting Page	1601
Ending Page	1606
Page Count	6
File Format	HTM / HTML PDF
ISSN	1744683X
Volume Number	5
Issue Number	8
Journal	Soft Matter
DOI	10.1039/b820385g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Mesenchymal stem cell Organic chemistry Thiol Carboxylic acid Tissue engineering Radical polymerization Addition reaction Covalent bond Matrix metalloproteinase Peptide
Content Type	Text
Resource Type	Article
Subject	Chemistry Condensed Matter Physics

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