NDLI: Cervicare™ induces apoptosis in HeLa and CaSki cells through ROS production and loss of mitochondrial membrane potential

Content Provider	Royal Society of Chemistry (RSC)
Author	Supriyanto, Eko Amini, Neda Jaganathan, Saravana Kumar Soon, Wong Tet Nasiri, Rozita Marvibaigi, Mohsen Hamzehalipour, Javad Majid, Fadzilah Adibah Abdul
Copyright Year	2016
Abstract	Cervicare™ is a poly-herbal preparation comprised of a combination of 6 plants; most have demonstrated antimicrobial and anticancer properties in preclinical studies. The effect of the ethanol and aqueous extracts of Cervicare™ on cell proliferation and apoptosis using cervical cancer HeLa and CaSki cells was investigated for the first time in the present study. MTT assay results showed that Cervicare™ extracts exerted time- and dose-dependent inhibition of cell viability. The hallmark properties of apoptosis like cell shrinkage and cytoplasmic condensation were observed using an inverted phase contrast microscope, ethidium bromide/acridine orange and Hoechst 33342/propidium iodide fluorescent staining methods. Furthermore, our results demonstrated that Cervicare™ extracts induced apoptosis in HeLa and CaSki cells by ROS generation and mitochondrial depolarization in a concentration dependent manner. The results showed that Cervicare™ extracts were capable of suppressing cell migration and inhibiting colony formation in a dose-dependent manner. Moreover, western blot analysis demonstrated the involvement of a mitochondria-dependent apoptosis pathway in the apoptosis inducing activity of Cervicare™ ethanol extract in HeLa cells. GC-MS analysis of the ethanolic extract afforded the identification of 40 substances, showing that it was primarily composed of anti-cancerous compounds such as xanthorrhizol (60.40%), octacosane (9.93%) and squalene (1.24%). Together, these results point out the Cervicare™ mediated inhibition of HeLa cell growth via induction of apoptosis and that it may be a potential anticancer agent which deserves further investigation.
Starting Page	24391
Ending Page	24417
Page Count	27
File Format	HTM / HTML PDF
ISSN	20462069
Volume Number	6
Issue Number	29
Journal	RSC Advances
DOI	10.1039/c5ra25654b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Antimicrobial Western blot Gas chromatography\u2013mass spectrometry Reactive oxygen species Ethanol Ant colony Squalene Propidium iodide MTT Hoechst MTT assay HeLa Acridine orange Depolarization Ethidium bromide Bisbenzimide Phase-contrast microscopy Cervical cancer Apoptosis
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering

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