Please wait, while we are loading the content...
| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Gupta, Prerak Kumar, Manishekhar Nandi, Samit K. Jain, Deepak Bhardwaj, Nandana Mandal, Biman B. |
| Copyright Year | 2016 |
| Abstract | Biomimetic natural origin biomaterials are noteworthy targets for further innovation in biomedical and tissue engineering. In this study, honeybee silk membranes (HBSM) are investigated in their native form to explore their applicability for tissue engineering. HBSMs extracted from honeybee combs were physico-chemically characterized for their surface topography, stability followed by evaluation of the biodegradation, mechanical and biological properties. Field emission scanning electron microscopy (FESEM) and atomic force microscopy (AFM) studies revealed a uniform sheet-like morphology with sub-micron pores and the presence of evenly knitted fibers in a specific pattern/alignment. Fourier transform infrared (FTIR) spectroscopy suggested the presence of a native coiled coil structural conformation. HBSMs were found to be cytocompatible and supported the proliferation of murine L929 fibroblasts, human osteosarcoma MG-63 cells and primary porcine knee chondrocytes. Cells cultured on HBSMs maintained osteogenic and chondrogenic potential as indicated by mineralization and accumulation of sulphated glycosaminoglycans (GAGs), respectively. In vitro analysis of the immune response (in terms of TNF-α release) and blood compatibility (in terms of LDH activity) further attests its possible applicability. Moreover, an in vivo subcutaneous implantation study in mice showed minimal inflammation. Taken together, this study demonstrates the potential of natural, biocompatible HBSMs as a suitable biomaterial for tissue engineering and regenerative medicine. |
| Starting Page | 54394 |
| Ending Page | 54403 |
| Page Count | 10 |
| File Format | HTM / HTML PDF |
| ISSN | 20462069 |
| Volume Number | 6 |
| Issue Number | 59 |
| Journal | RSC Advances |
| DOI | 10.1039/c6ra10738a |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Open |
| Subject Keyword | Biodegradation Minas Gerais Infrared spectroscopy Spectroscopy Tissue engineering Regenerative medicine Fourier transform Lactate dehydrogenase Inflammation Scanning electron microscope Coiled coil Silk Tumor necrosis factor alpha Fourier Atomic-force microscopy Osteosarcoma Biocompatibility Biomaterial Honey bee Protein structure |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Chemical Engineering |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|