NDLI: One-pot green synthesis of eumelanin: process optimization and its characterization

Content Provider	Royal Society of Chemistry (RSC)
Author	Saini, Amardeep Singh Melo, Jose Savio
Copyright Year	2015
Abstract	Advances in biocatalysis could help lower the environmental footprint of chemical processes. In this context, the present study reports for the first time the use of the Taguchi design of experiment methodology for optimizing the one-pot synthesis of eumelanin, using tyrosinase enzyme from Amorphophallus campanulatus and L-Dopa as the substrate. Using the Taguchi approach, an L27 orthogonal array was applied and five critical parameters/factors—pH, temperature, oxygen level, reaction time and enzyme units—were selected for the optimization. In order to maximize the response, the signal to noise (S/N) ratio related to the larger the better was used. The main effect and interaction plots of various factors were analyzed. Response tables and ANOVA revealed that pH is the most significant factor for melanin synthesis (P < 0.05). The yield of melanin and the rate of production under the best conditions were 1176 ± 3.2 mg L−1 and 24.5 mg L−1 h−1, respectively. Further, the biosynthesized melanin was characterized using various analytical techniques. The empirical formula of the synthesized melanin was found to be C8H8O4N. Based on the elemental analysis and A650/A500 ratio, the biosynthesized melanin was determined to be indole-type eumelanin. Thus, the present study describes the means to increase the yield of melanin.
Starting Page	47671
Ending Page	47680
Page Count	10
File Format	HTM / HTML PDF
ISSN	20462069
Volume Number	5
Issue Number	59
Journal	RSC Advances
DOI	10.1039/c5ra01962a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	One-pot synthesis Oxygen Biocatalysis Empirical formula Enzyme Orthogonality Amiga 500 ANOVA L-DOPA Taguchi A650 road Amorphophallus Mathematical optimization Melanin Tyrosinase Analysis of variance
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering

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L-DOPA Synthesis Using Tyrosinase Enzyme Precipitate Coatings on the Surface of Electrode

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Inductio n of pigmentation in mouse fibroblasts by expression of human tyrosinase (1989).

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One-pot green synthesis of eumelanin: process optimization and its characterization

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