NDLI: Towards building artificial light harvesting complexes: enhanced singlet–singlet energy transfer between donor and acceptor pairs bound to albumins

Please wait, while we are loading the content...

Supramolecular complexation and photochirogenesis with inherently chiral molecular clip: enantiodifferentiating photoisomerization of (Z,Z)-1,3-cyclooctadiene and polar photoaddition to 1,1-diphenylpropene

Photoinduced charge separation in pyrenedicarboxamide-linked DNA hairpins

Photophysical study of bay substituted perylenediimides

Towards building artificial light harvesting complexes: enhanced singlet–singlet energy transfer between donor and acceptor pairs bound to albumins

Inorganic photochemical protein scissors: photocleavage of lysozyme by Co(III) complexes

Kinetics of geminate recombination of organic free radicals in viscous solvents

Direct and water-mediated excited state intramolecular proton transfer (ESIPT) from phenol OH to carbon atoms of extended ortho-substituted biaryl systems

Consequences of controlling free space within a reaction cavity with a remote alkyl group: photochemistry of para-alkyl dibenzyl ketones within an organic capsule in water

Biocidal performance of acrylated glyphosate in a model photopolymerizable coating formulation

Synthesis and photo-conversion of androsta- and pregna-5,7-dienes to vitamin D3-like derivatives

A yellow chlorophyll catabolite is a pigment of the fall colours

Back matter

Back cover

Photochemical & Photobiological Sciences Vol - 7 Issue - 9

Photochemical & Photobiological Sciences Vol - 7 Issue - 8

Photochemical & Photobiological Sciences Vol - 7 Issue - 7

Photochemical & Photobiological Sciences Vol - 7 Issue - 6

Photochemical & Photobiological Sciences Vol - 7 Issue - 5

Photochemical & Photobiological Sciences Vol - 7 Issue - 4

Photochemical & Photobiological Sciences Vol - 7 Issue - 3

Photochemical & Photobiological Sciences Vol - 7 Issue - 2

Photochemical & Photobiological Sciences Vol -6

Photochemical & Photobiological Sciences Vol -5

Photochemical & Photobiological Sciences Vol -4

Photochemical & Photobiological Sciences Vol -3

Photochemical & Photobiological Sciences Vol -2

Towards building artificial light harvesting complexes: enhanced singlet–singlet energy transfer between donor and acceptor pairs bound to albumins

Content Provider	Royal Society of Chemistry (RSC)
Author	Kumar, Challa V. Duff, Michael R.
Copyright Year	2008
Abstract	Specific donor and acceptor pairs have been assembled in bovine serum albumin (BSA), at neutral pH and room temperature, and these dye–protein complexes indicated efficient donor to acceptor singlet–singlet energy transfer. For example, pyrene-1-butyric acid served as the donor and Coumarin 540A served as the acceptor. Both the donor and the acceptor bind to BSA with affinity constants in excess of 2 × 105 M−1, as measured in absorption and circular dichroism (CD) spectral titrations. Simultaneous binding of both the donor and the acceptor chromophores was supported by CD spectra and one chromophore did not displace the other from the protein host, even when limited concentrations of the host were used. For example, a 1:1:1 complex between the donor, acceptor and the host can be readily formed, and spectral data clearly show that the binding sites are mutually exclusive. The ternary complexes (two different ligands bound to the same protein molecule) provided opportunities to examine singlet–singlet energy transfer between the protein-bound chromophores. Donor emission was quenched by the addition of the acceptor, in the presence of limited amounts of BSA, while no energy transfer was observed in the absence of the protein host, under the same conditions. The excitation spectra of the donor–acceptor-host complexes clearly show the sensitization of acceptor emission by the donor. Protein denaturation, as induced by the addition of urea or increasing the temperature to 360 K, inhibited energy transfer, which indicate that protein structure plays an important role. Sensitization also proceeded at low temperature (77 K) and diffusion of the donor or the acceptor is not required for energy transfer. Stern–Volmer quenching plots show that the quenching constant is (3.1 ± 0.2) × 104 M−1, at low acceptor concentrations (<35 μM). Other albumins such as human and porcine proteins also served as good hosts for the above experiments. For the first time, non-natural systems have been self-assembled which can capture donor–acceptor pairs and facilitate singlet–singlet energy transfer. Such systems may form a basis for the design and construction of protein-based multi-chromophore self-assemblies for solar light harvesting, conversion and storage.
Starting Page	1522
Ending Page	1530
Page Count	9
File Format	HTM / HTML PDF
ISSN	1474905X
Volume Number	7
Issue Number	12
Journal	Photochemical & Photobiological Sciences
DOI	10.1039/b811091c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Bovine serum albumin Volmer Coumarin Protein Circular dichroism BSA Pyrene Urea Denaturation (biochemistry) Diffusion Protein structure Chromophore
Content Type	Text
Resource Type	Article
Subject	Physical and Theoretical Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Spectral study of coumarin-3-carboxylic acid interaction with human and bovine serum albumins

Pressure- and Urea-Induced Denaturation of Bovine Serum Albumin: Considerations about Protein Heterogeneity

Licochalcone A bound to bovine serum albumin: a spectroscopic, photophysical and structural study

Characterization of the interaction between eupatorin and bovine serum albumin by spectroscopic and molecular modeling methods (2013).

A DSC study of zinc binding to bovine serum albumin (BSA)

Novel Bovine Serum Albumin Protein Backbone Reassembly Study: Strongly Twisted β-Sheet Structure Promotion upon Interaction with GO-PAMAM

Interaction of Merocyanine 540 with serum albumins: photophysical and binding studies.

A DSC study of zinc binding to bovine serum albumin (BSA)

A DSC study of zinc binding to bovine serum albumin (BSA)

Towards building artificial light harvesting complexes: enhanced singlet–singlet energy transfer between donor and acceptor pairs bound to albumins

Similar Documents

Spectral study of coumarin-3-carboxylic acid interaction with human and bovine serum albumins

Pressure- and Urea-Induced Denaturation of Bovine Serum Albumin: Considerations about Protein Heterogeneity

Licochalcone A bound to bovine serum albumin: a spectroscopic, photophysical and structural study

Characterization of the interaction between eupatorin and bovine serum albumin by spectroscopic and molecular modeling methods (2013).

A DSC study of zinc binding to bovine serum albumin (BSA)

Novel Bovine Serum Albumin Protein Backbone Reassembly Study: Strongly Twisted β-Sheet Structure Promotion upon Interaction with GO-PAMAM

Interaction of Merocyanine 540 with serum albumins: photophysical and binding studies.

A DSC study of zinc binding to bovine serum albumin (BSA)

A DSC study of zinc binding to bovine serum albumin (BSA)

Towards building artificial light harvesting complexes: enhanced singlet–singlet energy transfer between donor and acceptor pairs bound to albumins