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| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Sommaruga, Ruben Hörtnagl, Paul H. |
| Copyright Year | 2007 |
| Abstract | We tested the hypothesis that photo-oxidative stress is greater in symbiotic representatives of the freshwater ciliate Paramecium bursaria than in aposymbiotic (i.e., without Chlorella) ones. The level of oxidative stress was determined by assessing reactive oxygen species (ROS) with two fluorescent probes (hydroethidine and dihydrorhodamine123) by flow cytometry in exponential and stationary growth phases of both strains. Photo-oxidative stress was assessed in the laboratory after exposure of the ciliates to photosynthetically active radiation (PAR: 400–700 nm) and PAR + ultraviolet radiation (UVR: 280–400 nm). Additionally, both strains were screened for their antioxidant defenses by measuring the activity of the enzymes catalase, superoxide dismutase (SOD), and glutathione reductase. The results showed that aposymbiotic ciliates had higher levels of PAR-induced oxidative stress than symbiotic ones. Significant differences in PAR-induced oxidative stress were also found in both strains when comparing exponential and stationary growth phases with generally higher values in the former. After exposure to UVR, aposymbiotic ciliates in the stationary phase had the highest levels of ROS despite an increase in SOD activity. By contrast, exposure to UVR decreased catalase activity in both strains. Overall, our results suggest that in this ciliate symbiosis, the presence of symbionts minimizes photo-oxidative stress. This work represents the first assessment of photo-oxidative stress in an algal-ciliate mutualistic symbiosis. |
| Starting Page | 842 |
| Ending Page | 847 |
| Page Count | 6 |
| File Format | HTM / HTML PDF |
| ISSN | 1474905X |
| Volume Number | 6 |
| Issue Number | 8 |
| Journal | Photochemical & Photobiological Sciences |
| DOI | 10.1039/b703119j |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Subscribed |
| Subject Keyword | Symbiosis Oxidative stress Flow cytometry Chlorella Reactive oxygen species Photosynthetically active radiation Aposymbiotic Superoxide dismutase Ultraviolet Antioxidant UVR Bacterial growth Paramecium bursaria Catalase Glutathione reductase Beetle Ciliate |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physical and Theoretical Chemistry |
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