NDLI: Rapid determination of seed vigor based on the level of superoxide generation during early imbibition

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Photochemical & Photobiological Sciences Vol - 6 Issue - 6

Photochemical & Photobiological Sciences Vol - 6 Issue - 5

Photochemical & Photobiological Sciences Vol - 6 Issue - 4

Photochemical & Photobiological Sciences Vol - 6 Issue - 3

Photochemical & Photobiological Sciences Vol - 6 Issue - 2

Photochemical & Photobiological Sciences Vol -5

Photochemical & Photobiological Sciences Vol -4

Photochemical & Photobiological Sciences Vol -3

Photochemical & Photobiological Sciences Vol -2

Rapid determination of seed vigor based on the level of superoxide generation during early imbibition

Content Provider	Royal Society of Chemistry (RSC)
Author	Xing, Da Zhang, Lingrui Liu, Xuejun Li, Lingling
Copyright Year	2007
Abstract	It has been reported that a large amount of reactive oxygen species (ROS) is produced during seed imbibition and this ROS is related to seed vigor. To make this physiological mechanism clear, we have used 2-methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo(1,2-α)pyrazin-3-one (MCLA) as a sensitive and physiologically compatible probe for the determination of superoxide anion (O2˙–) production in vivo. Our results showed that dry rice (Oryzae sativa L.) seed embryo cells possessed the capacity to generate O2˙–. Conversely, the O2˙– production of seed embryo cells was inhibited by quinacrine (QA) and diphenylene iodonium (DPI), two specific inhibitors of NADPH oxidase, and O2˙– induced MCLA-mediated chemiluminescence was also blocked by superoxide dismutase (SOD). Additionally, O2˙–-production ability increased dramatically in a NADPH-dependent way in the plasma membraneprotein abstract from rice seed embryo cells, whereas SOD and the inhibitors mentioned above suppressed O2˙– production. These preliminary results suggested that rice seeds contained intrinsic NADPH oxidase activity. To validate this conclusion, dichlorofluorescein (DCF) fluorescence staining was used (observed under a laser scanning microscope, LSM) to reflect the in situ assessment of O2˙–-generation. The position of O2˙– production located at the plasma membrane. Additionally the ability to synthesize O2˙– was activated directly by calcium ions. These observations are in accord with the character of NADPH oxidase catalyzed O2˙–-generation. All these results indicated that NADPH oxidase contribute to O2˙– production and release to the outside. We concluded that NADPH oxidase plays an intrinsic role as an NADPH sensor, so, measuring the O2˙– one can monitor the NADPH concentration, which is an index of seed vigor. Therefore the O2˙– generation during early imbibition can serve as a rapid measurement of seed vigor.
Starting Page	767
Ending Page	774
Page Count	8
File Format	HTM / HTML PDF
ISSN	1474905X
Volume Number	6
Issue Number	7
Journal	Photochemical & Photobiological Sciences
DOI	10.1039/b704337f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	NADPH Reactive oxygen species Oxygen Embryo Cell membrane Calcium Imbibition Fluorescence Ion Superoxide dismutase Superoxide Chemiluminescence MCLA Oryza sativa Dichlorofluorescein Microscopy Nicotinamide adenine dinucleotide phosphate NADPH oxidase Laser scanning Mepacrine
Content Type	Text
Resource Type	Article
Subject	Physical and Theoretical Chemistry

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