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Quality control of Photosystem II: an FtsH protease plays an essential role in the turnover of the reaction center D1 protein in Synechocystis PCC 6803 under heat stress as well as light stress conditions

Mutagenesis of CP43-arginine-357 to serine reveals new evidence for (bi)carbonate functioning in the water oxidizing complex of Photosystem II

Conformational changes and their role in non-radiative energy dissipation in photosystem II reaction centres

Structure and evolution of the extrinsic proteins that stabilize the oxygen-evolving engine

Solving the structure of plant photosystem I—biochemistry is vital

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy

The assembly and organisation of photosynthetic membranes in Rhodobacter sphaeroides

Isolation and characterization of a xanthophyll-rich fraction from the thylakoid membrane of Dunaliella salina(green algae)

The mechanism for dioxygen formation in PSII studied by quantum chemical methods

A role of the C-terminal extension of the photosystem II D1 protein in sensitivity of the cyanobacterium Synechocystis PCC 6803 to photoinhibition

The role of D1-Ala344 in charge stabilization and recombination in Photosystem II

Kinetic characterisation of the light-driven protochlorophyllide oxidoreductase (POR) from Thermosynechococcus elongatus

The nature of the photosystem II reaction centre in the chlorophyll d-containing prokaryote, Acaryochloris marina

Degradation of the main Photosystem II light-harvesting complex

Functional flexibility and acclimation of the thylakoid membrane

Granal stacking of thylakoid membranes in higher plant chloroplasts: the physicochemical forces at work and the functional consequences that ensue

Photosystem I: a search for green plant trimers

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Photochemical & Photobiological Sciences Vol - 4 Issue - 9

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Photochemical & Photobiological Sciences Vol -2

The role of D1-Ala344 in charge stabilization and recombination in Photosystem II

Content Provider	Royal Society of Chemistry (RSC)
Author	Diner, Bruce A. Nixon, Peter J. Cser, Krisztián Vass, Imre
Copyright Year	2005
Abstract	The Ala344 residue of the D1 protein has been identified as a crucial residue of the catalytic cluster of the water-oxidizing complex, however, its function has not been fully clarified. Here we have used thermoluminescence and flash-induced chlorophyll fluorescence measurements to characterize the effect of the D1-Ala344stop mutation on the electron transport of Photosystem II in intact cells of the cyanobacterium Synechocystis 6803. Although the mutant cannot grow photoautotrophically it shows flash-induced thermoluminescence and chlorophyll fluorescence signals reflecting the stabilization of negative and positive charges on the QA and QB quinone electron acceptors, and stable Photosystem II donors, respectively. Decay of flash induced chlorophyll fluorescence yield is multiphasic in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), with 6 ms, 350 ms, and 26 s time constants. When cells are illuminated with repetitive flashes, fired at 1 ms intervals, the 6 ms phase is gradually decreased with the concomitant increase of the 350 ms phase. After 45 min dark adaptation of mutant cells the 6 ms and 350 ms phases were significantly decreased and a very slow decaying component was formed. Flash induced oscillation of the thermoluminescence B band, which reflects the redox cycling of the water-oxidizing complex in the wild-type cells, was completely abolished in the D1-Ala344stop mutant. The results demonstrate that low efficiency photooxidation of Mn occurs in about 60% of the PSII centers. The photooxidizable Mn is unstable in the dark, and formation of higher S states is inhibited. In addition, the QA to QB electron transfer step is slowed down as an indirect consequence of the donor side modification. Our data indicate that the stabilization of a Mn ion by the α-carboxylate chain of the D1-Ala344 residue might represent one of the final steps in the assembly of functional catalytic sites for water oxidation.
Starting Page	1049
Ending Page	1054
Page Count	6
File Format	HTM / HTML PDF
ISSN	1474905X
Volume Number	4
Issue Number	12
Journal	Photochemical & Photobiological Sciences
DOI	10.1039/b512354m
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Thermoluminescence dating PSII Quinone Cyanobacteria Fluorescence Redox Ion Photosystem II Synechocystis Mutation Adaptation (eye) Protein
Content Type	Text
Resource Type	Article
Subject	Physical and Theoretical Chemistry

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A role of the C-terminal extension of the photosystem II D1 protein in sensitivity of the cyanobacterium Synechocystis PCC 6803 to photoinhibition

A Transient Exchange of the Photosystem II Reaction Center Protein D1:1 with D1:2 during Low Temperature Stress ofSynechococcus sp. PCC 7942 in the Light Lowers the Redox Potential of QB

Engineering of n-terminal threonines in the d1 protein impairs photosystem ii energy transfer in synechocystis 6803 (1998).

Radiative and non-radiative charge recombination pathways in Photosystem II studied by thermoluminescence and chlorophyll fluorescence in the cyanobacterium Synechocystis 6803.

Mutagenesis of CP43-arginine-357 to serine reveals new evidence for (bi)carbonate functioning in the water oxidizing complex of Photosystem II

Charge recombination and thermoluminescence in photosystem II.

In vivo quality control of photosystem II in cyanobacteria Synechocystis sp. PCC 6803: D1 protein degradation and repair under the influence of light, heat and darkness

The function of D1-H332 in Photosystem II electron transport studied by thermoluminescence and chlorophyll fluorescence in site-directed mutants of Synechocystis 6803

Variants of photosystem II D1 protein in Thermosynechococcus elongatus

The role of D1-Ala344 in charge stabilization and recombination in Photosystem II

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A role of the C-terminal extension of the photosystem II D1 protein in sensitivity of the cyanobacterium Synechocystis PCC 6803 to photoinhibition

A Transient Exchange of the Photosystem II Reaction Center Protein D1:1 with D1:2 during Low Temperature Stress ofSynechococcus sp. PCC 7942 in the Light Lowers the Redox Potential of QB

Engineering of n-terminal threonines in the d1 protein impairs photosystem ii energy transfer in synechocystis 6803 (1998).

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In vivo quality control of photosystem II in cyanobacteria Synechocystis sp. PCC 6803: D1 protein degradation and repair under the influence of light, heat and darkness

The function of D1-H332 in Photosystem II electron transport studied by thermoluminescence and chlorophyll fluorescence in site-directed mutants of Synechocystis 6803

Variants of photosystem II D1 protein in Thermosynechococcus elongatus

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