NDLI: Sigmoidal reduction kinetics of the photosystem II acceptor side in intact photosynthetic materials during fluorescence induction

Content Provider	Royal Society of Chemistry (RSC)
Author	Carpentier, Robert Joly, David
Copyright Year	2009
Abstract	Illumination of dark-adapted photosynthetic samples leads to fluorescence induction (FI) that can be described by a triphasic O-J-I-P fluorescence rise. Its kinetics follows the accumulation of reduced photosystem II (PSII) acceptors. In isolated thylakoid membranes, FI is often used to study photosynthetic electron transport. A simple quantitative analysis method was recently developed to fit these FI traces and also lead to a better understanding of action sites of artificial electron acceptors. However, a quantitative method was still lacking for FI in intact systems like leaves, where the FI kinetics shows a clear I-peak. Here, we present a new quantitative method to analyze experimental FI traces in leaves and intact chloroplasts. It revealed a sigmoidicity in the reduction kinetics of the PSII acceptor side of intact systems. The results also show that the origin of each phase is independent of the photosynthetic material used. The effects of decyl-plastoquinone on intact chloroplasts retarded predominantly the I-P rise and clearly indicates that this phase is related to the accumulation of a reduce PQ pool, as observed in isolated thylakoid membranes.
Starting Page	167
Ending Page	173
Page Count	7
File Format	HTM / HTML PDF
ISSN	1474905X
Volume Number	8
Issue Number	2
Journal	Photochemical & Photobiological Sciences
DOI	10.1039/b815070b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Photosynthetic reaction centre PSII Fluorescence Photosystem II Thylakoid
Content Type	Text
Resource Type	Article
Subject	Physical and Theoretical Chemistry

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