NDLI: A new FRET-based ratiometric probe for fluorescence and colorimetric analyses of adenosine 5′-triphosphate

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A new FRET-based ratiometric probe for fluorescence and colorimetric analyses of adenosine 5′-triphosphate

Content Provider	Royal Society of Chemistry (RSC)
Author	Liu, Cui Lu, Yan An, Nianqi Zhang, Qiang Shi, Luqing Wang, Jing Liu, Lihua Deng, Ludi
Copyright Year	2017
Abstract	This paper reports a new ratiometric probe for the detection of adenosine 5′-triphosphate (ATP) that is based on the binding-induced modulation of fluorescence resonance energy transfer (FRET) coupled with the aggregation-caused quenching (ACQ) sensing mechanism. The introduction of coumarin fluorophores as FRET donors into the side-chain of polythiophene (PT) as the FRET acceptor provides the ratiometric probe. Upon addition of ATP, the formation of a polymer/ATP complex leads to the conformational changes of the PT backbone from a random coil to the planar conformation and aggregation. As such, the ratiometric ATP detection can be achieved by taking advantage of spectral overlap changes to modulate the FRET efficiency from coumarin fluorophores to the PT backbone before and after the addition of ATP, coupled with ACQ. The probe exhibited a clear seesaw-type dual-emission signal change upon binding with strong affinity (Kapp = 3.12 × 104 M−1) to ATP in aqueous solution, whereas no obvious emission changes were observed with ADP, AMP or various other anions. The probe can also be utilized for naked-eye detection of ATP and real-time monitoring of the ATP hydrolysis reaction by apyrase under physiological pH conditions.
Starting Page	1138
Ending Page	1145
Page Count	8
File Format	HTM / HTML PDF
ISSN	17599954
Volume Number	8
Issue Number	7
Journal	Polymer Chemistry
DOI	10.1039/c6py02001a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Förster resonance energy transfer Adenosine monophosphate Polythiophene Acq, Pas-de-Calais Fluorescence Polymer Coumarin Conformational change ACQ Hydrolysis Kapp Records Polyphosphate Kapp Apyrase Random coil ATP Adenosine triphosphate
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry Bioengineering Biomedical Engineering Polymers and Plastics

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