NDLI: Synthesis of guanosine 5′-conjugates and their use as initiator molecules for transcription priming

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Organic & Biomolecular Chemistry Vol - 6 Issue - 4

Organic & Biomolecular Chemistry Vol - 6 Issue - 3

Organic & Biomolecular Chemistry Vol -5

Organic & Biomolecular Chemistry Vol -4

Organic & Biomolecular Chemistry Vol -3

Organic & Biomolecular Chemistry Vol -2

Synthesis of guanosine 5′-conjugates and their use as initiator molecules for transcription priming

Content Provider	Royal Society of Chemistry (RSC)
Author	Dombos, Valeska Appel, Bettina Wolf, Jörn Müller, Sabine
Copyright Year	2008
Abstract	We have synthesised two guanosine derivatives that are linked to biotinylated adenosine moieties by using two different strategies, one that includes synthetic steps on the solid phase and another one that is performed entirely in solution. The synthesised derivatives were shown to function as initiator molecules in transcription priming experiments. The incorporation efficiency was determined to be approximately 2%. Even though this value is rather low, the use of either molecule in selection experiments seems reasonable. Basically, RNA libraries with sequence complexities of 1015 to 1016 can be generated. Labelling of such a library with our initiator molecule would still produce 1013 to 1014 labelled/functionalised sequences, and thus sufficient sequence space for selection.
Starting Page	899
Ending Page	907
Page Count	9
File Format	HTM / HTML PDF
ISSN	14770520
Volume Number	6
Issue Number	5
Journal	Organic & Biomolecular Chemistry
DOI	10.1039/b716151d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Adenosine RNA Guanosine Library Sequence space
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry Physical and Theoretical Chemistry

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