NDLI: Acetylcholinesterase inhibition-based colorimetric determination of Hg<sup>2+</sup> using unmodified silver nanoparticles

Content Provider	Royal Society of Chemistry (RSC)
Author	Kumar, Dashananda Nanda Rajeshwari, Ayyachamy Chandrasekaran, Natarajan Alex, Sruthi Ann Mukherjee, Amitava
Copyright Year	2015
Abstract	A novel, enzyme-based, indirect detection method for mercury in aqueous solutions has been developed using unmodified silver nanoparticles. The enzyme acetylcholinesterase breaks down acetylthiocholine to thiocholine, which results in the crosslinking of the silver nanoparticles. The presence of mercury in solution prevents the aggregation of the silver nanoparticles by reversibly inhibiting the active site of the enzyme. The UV-visible absorption peak at 393 nm for the unmodified silver nanoparticles was found to decrease in the presence of acetylcholinesterase and acetylthiocholine. Upon adding mercury to the system, a concentration-dependent increase in the primary peak intensity at 393 nm was noted. The designed system was found to have good linearity (R2 = 0.9799) in the range of 10 to 50 nM of mercury with an excellent limit of detection of 1.18 × 10−9 M in aqueous solution. This probe was successfully employed for the detection of mercury in spiked lakewater, groundwater, and seawater samples.
Starting Page	1172
Ending Page	1178
Page Count	7
File Format	HTM / HTML PDF
ISSN	11440546
Volume Number	39
Issue Number	2
Journal	New Journal of Chemistry
DOI	10.1039/c4nj01722f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Groundwater Enzyme Active site Mercury-vapor lamp Acetylcholinesterase
Content Type	Text
Resource Type	Article
Subject	Chemistry Materials Chemistry Catalysis

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