NDLI: Insight into the mechanism revealing the peroxidase mimetic catalytic activity of quaternary CuZnFeS nanocrystals: colorimetric biosensing of hydrogen peroxide and glucose

Content Provider	Royal Society of Chemistry (RSC)
Author	Acharya, Somobrata Pradhan, Bapi Khan, Ali Hossain Kumar, Gundam Sandeep Ghosh, Tanmay Satpati, Biswarup Thupakula, Umamahesh Dalui, Amit
Copyright Year	2015
Abstract	Artificial enzyme mimetics have attracted immense interest recently because natural enzymes undergo easy denaturation under environmental conditions restricting practical usefulness. We report for the first time chalcopyrite CuZnFeS (CZIS) alloyed nanocrystals (NCs) as novel biomimetic catalysts with efficient intrinsic peroxidase-like activity. Novel peroxidase activities of CZIS NCs have been evaluated by catalytic oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H2O2). CZIS NCs demonstrate the synergistic effect of elemental composition and photoactivity towards peroxidase-like activity. The quaternary CZIS NCs show enhanced intrinsic peroxidase-like activity compared to the binary NCs with the same constituent elements. Intrinsic peroxidase-like activity has been correlated with the energy band position of CZIS NCs extracted using scanning tunneling spectroscopy and ultraviolet photoelectron spectroscopy. Kinetic analyses indicate Michaelis–Menten enzyme kinetic model catalytic behavior describing the rate of the enzymatic reaction by correlating the reaction rate with substrate concentration. Typical color reactions arising from the catalytic oxidation of TMB over CZIS NCs with H2O2 have been utilized to establish a simple and sensitive colorimetric assay for detection of H2O2 and glucose. CZIS NCs are recyclable catalysts showing high efficiency in multiple uses. Our study may open up the possibility of designing new photoactive multi-component alloyed NCs as enzyme mimetics in biotechnology applications.
Starting Page	9062
Ending Page	9074
Page Count	13
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	7
Issue Number	19
Journal	Nanoscale
DOI	10.1039/c5nr01728a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Biotechnology Hydrogen peroxide Enzyme TMB Michaelis Photoemission spectroscopy Glucose Reaction rate Chalcopyrite Biomimetics Artificial enzyme Peroxidase Scanning tunneling spectroscopy Denaturation (biochemistry) Ultraviolet photoelectron spectroscopy
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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