NDLI: Smart polymer brush nanostructures guide the self-assembly of pore-spanning lipid bilayers with integrated membrane proteins

Content Provider	Royal Society of Chemistry (RSC)
Author	Demarche, Sophie Vancso, G. Julius Groot, G. Wilhelmina de Tiefenauer, Louis Santonicola, M. Gabriella
Copyright Year	2014
Abstract	Nanopores in arrays on silicon chips are functionalized with pH-responsive poly(methacrylic acid) (PMAA) brushes and used as supports for pore-spanning lipid bilayers with integrated membrane proteins. Robust platforms are created by the covalent grafting of polymer brushes using surface-initiated atom transfer radical polymerization (ATRP), resulting in sensor chips that can be successfully reused over several assays. His-tagged proteins are selectively and reversibly bound to the nitrilotriacetic acid (NTA) functionalization of the PMAA brush, and consequently lipid bilayer membranes are formed. The enhanced membrane resistance as determined by electrochemical impedance spectroscopy and free diffusion of dyed lipids observed as fluorescence recovery after photobleaching confirmed the presence of lipid bilayers. Immobilization of the His-tagged membrane proteins on the NTA-modified PMAA brush near the pore edges is characterized by fluorescence microscopy. This system allows us to adjust the protein density in free-standing bilayers, which are stabilized by the polymer brush underneath. The potential application of the integrated platform for ion channel protein assays is demonstrated.
Starting Page	2228
Ending Page	2237
Page Count	10
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	6
Issue Number	4
Journal	Nanoscale
DOI	10.1039/c3nr05356c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Atom-transfer radical-polymerization Spectroscopy Poly(methacrylic acid) Fluorescence Polymer brush Polymer Ion Fluorescence microscope Grafting Lipid Protein PMAA Covalent bond Lipid bilayer Electrochemistry NTA Diffusion Electrical impedance Photobleaching
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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