NDLI: Gold nanoparticles induce transcriptional activity of NF-κB in a B-lymphocyte cell line

Please wait, while we are loading the content...

Mechanically resilient electrospun TiC nanofibrous mats surface-decorated with Pt nanoparticles for oxygen reduction reaction with enhanced electrocatalytic activities

Gas-assisted growth of boron-doped nickel nanotube arrays: rapid synthesis, growth mechanisms, tunable magnetic properties, and super-efficient reduction of 4-nitrophenol

Atomistic simulations of highly conductive molecular transport junctions under realistic conditions

One-pot fabrication of single-crystalline octahedral Pt–Cu nanoframes and their enhanced electrocatalytic activity

High responsivity, fast ultraviolet photodetector fabricated from ZnO nanoparticle–graphene core–shell structures

Atomically precise Au₂₅ superatoms immobilized on CeO₂ nanorods for styrene oxidation

Demonstration of specific binding of heparin to Plasmodium falciparum-infected vs. non-infected red blood cells by single-molecule force spectroscopy

Coarse-grained molecular dynamics simulations of photoswitchable assembly and disassembly

Self-catalytic solution–liquid–liquid–solid (SLLS) growth of tapered SnS nanorods

Digital selective growth of a ZnO nanowire array by large scale laser decomposition of zinc acetate

Defect effects on the physical and electrochemical properties of nanoscale LiFe_0.92PO₄ and LiFe_0.92PO₄/C/graphene composites

Core–shell designs of photoluminescent nanodiamonds with porous silica coatings for bioimaging and drug delivery II: application

Surface charge of polymer coated SPIONs influences the serum protein adsorption, colloidal stability and subsequent cell interaction in vitro

Surface-modified multifunctional MIP nanoparticles

Fabrication of Au nanotube arrays and their plasmonic properties

Gold nanoparticles induce transcriptional activity of NF-κB in a B-lymphocyte cell line

Band structure quantization in nanometer sized ZnO clusters

Transfer of highly porous nanoparticle layers to various substrates through mechanical compression

Graphene oxide embedded sandwich nanostructures for enhanced Raman readout and their applications in pesticide monitoring

Strong composite films with layered structures prepared by casting silk fibroin–graphene oxide hydrogels

Transparent nanopaper with tailored optical properties

Preparation of Fe₃O₄ with high specific surface area and improved capacitance as a supercapacitor

Cationic poly(lactic-co-glycolic acid) iron oxide microspheres for nucleic acid detection

TMV nanorods with programmed longitudinal domains of differently addressable coat proteins

Gold nanoparticle-enhanced luminescence of silicon quantum dots co-encapsulated in polymer nanoparticles

Type-II ZnO nanorod–SnO₂ nanoparticle heterostructures: characterization of structural, optical and photocatalytic properties

The potent antimicrobial properties of cell penetrating peptide-conjugated silver nanoparticles with excellent selectivity for Gram-positive bacteria over erythrocytes

Enhanced broadband and omnidirectional performance of Cu(In,Ga)Se₂ solar cells with ZnO functional nanotree arrays

Graphene oxide-encapsulated carbon nanotube hybrids for high dielectric performance nanocomposites with enhanced energy storage density

Carbon nanotube-templated polyaniline nanofibers: synthesis, flash welding and ultrafiltration membranes

Escape from the destruction of the galvanic replacement reaction for solid → hollow → solid conversion process in one pot reaction

One-pot formation of multifunctional Pt-conducting polymer intercalated nanostructures

Efficient electron and hole doping in compositionally abrupt Si/Ge nanowires

Giant optical activity from the radiative electromagnetic interactions in plasmonic nanoantennas

Development of drug loaded nanoparticles for tumor targeting. Part 1: synthesis, characterization, and biological evaluation in 2D cell cultures

Development of drug loaded nanoparticles for tumor targeting. Part 2: Enhancement of tumor penetration through receptor mediated transcytosis in 3D tumor models

Hierarchical metallic and ceramic nanostructures from laser interference ablation and block copolymer phase separation

Dual-donor (Zn_i and V_O) mediated ferromagnetism in copper-doped ZnO micron-scale polycrystalline films: a thermally driven defect modulation process

Targeting polymeric fluorescent nanodiamond-gold/silver multi-functional nanoparticles as a light-transforming hyperthermia reagent for cancer cells

Magnetic properties of cylindrical diameter modulated Ni₈₀Fe₂₀ nanowires: interaction and coercive fields

Nanocomposites of silver nanoparticles embedded in glass nanofibres obtained by laser spinning

Chemical insight into electroforming of resistive switching manganite heterostructures

Controlling potassium selectivity and proton blocking in a hybrid biological/solid-state polymer nanoporous membrane

Structural diversity in iron oxide nanoparticle assemblies as directed by particle morphology and orientation

Electroactive nanoparticle directed assembly of functionalized graphene nanosheets into hierarchical structures with hybrid compositions for flexible supercapacitors

Multidentate zwitterionic chitosan oligosaccharide modified gold nanoparticles: stability, biocompatibility and cell interactions

Inside back cover

Back cover

Nanoscale Vol - 5 Issue - 8

Nanoscale Vol - 5 Issue - 7

Nanoscale Vol - 5 Issue - 6

Nanoscale Vol - 5 Issue - 5

Nanoscale Vol - 5 Issue - 4

Nanoscale Vol - 5 Issue - 3

Gold nanoparticles induce transcriptional activity of NF-κB in a B-lymphocyte cell line

Content Provider	Royal Society of Chemistry (RSC)
Author	Sharma, Monita Sulentic, Courtney E. W. Hussain, Saber M. Salisbury, Richard L. Maurer, Elizabeth I.
Copyright Year	2013
Abstract	Gold nanoparticles (Au-NPs) have been designated as superior tools for biological applications owing to their characteristic surface plasmon absorption/scattering and amperometric (electron transfer) properties, in conjunction with low or no immediate toxicity towards biological systems. Many studies have shown the ease of designing application-based tools using Au-NPs but the interaction of this nanosized material with biomolecules in a physiological environment is an area requiring deeper investigation. Immune cells such as lymphocytes circulate through the blood and lymph and therefore are likely cellular components to come in contact with Au-NPs. The main aim of this study was to mechanistically determine the functional impact of Au-NPs on B-lymphocytes. Using a murine B-lymphocyte cell line (CH12.LX), treatment with citrate-stabilized 10 nm Au-NPs induced activation of an NF-κB-regulated luciferase reporter, which correlated with altered B lymphocyte function (i.e. increased antibody expression). TEM imaging demonstrated that Au-NPs can pass through the cellular membrane and therefore could interact with intracellular components of the NF-κB signaling pathway. Based on the inherent property of Au-NPs to bind to –thiol groups and the presence of cysteine residues on the NF-κB signal transduction proteins IκB kinases (IKK), proteins specifically bound to Au-NPs were extracted from CH12.LX cellular lysate exposed to 10 nm Au-NPs. Electrophoresis identified several bands, of which IKKα and IKKβ were immunoreactive. Further evaluation revealed activation of the canonical NF-κB signaling pathway as evidenced by IκBα phosphorylation at serine residues 32 and 36 followed by IκBα degradation and increased nuclear RelA. Additionally, expression of an IκBα super-repressor (resistant to proteasomal degradation) reversed Au-NP-induced NF-κB activation. Altered NF-κB signaling and cellular function in B-lymphocytes suggests a potential for off-target effects with in vivo applications of gold nanomaterials and underscores the need for more studies evaluating the interactions of nanomaterials with biomolecules and cellular components.
Starting Page	3747
Ending Page	3756
Page Count	10
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	5
Issue Number	9
Journal	Nanoscale
DOI	10.1039/c3nr30071d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Lymphatic system Thiol Cysteine Phosphorylation Luciferase Interaction Serine Nanomaterials Surface plasmon IκB kinase IκBα B cell NF-κB Signal transduction Reporter gene Transmission electron microscopy Electrophoresis
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Hydrogen Peroxide Activates NF-κB through Tyrosine Phosphorylation of IκBα and Serine Phosphorylation of p65 EVIDENCE FOR THE INVOLVEMENT OF IκBα KINASE AND Syk PROTEIN-TYROSINE KINASE

Inhibition of Interleukin-1-stimulated NF-κB RelA/p65 Phosphorylation by Mesalamine Is Accompanied by Decreased Transcriptional Activity

Phosphorylation of Thr-516 and Ser-520 in the Kinase Activation Loop of MEKK3 Is Required for Lysophosphatidic Acid-mediated Optimal IκB Kinase β (IKKβ)/Nuclear Factor-κB (NF-κB) Activation

A20-binding Inhibitor of Nuclear Factor-κB (NF-κB)-2 (ABIN-2) Is an Activator of Inhibitor of NF-κB (IκB) Kinase α (IKKα)-mediated NF-κB Transcriptional Activity

Phosphorylation of RelA/p65 on Serine 536 Defines an IκBα-independent NF-κB Pathway

IκB Kinase β (IKBKB) Mutations in Lymphomas That Constitutively Activate Canonical Nuclear Factor κB (NFκB) Signaling

HSCARG inhibits activation of NF-κB by interacting with IκB kinase-β

IKKβ Is Required for Bcl-2-mediated NF-κB Activation in Ventricular Myocytes

3-Phosphoinositide-dependent Protein Kinase-1-mediated IκB Kinase β (IKKB) Phosphorylation Activates NF-κB Signaling

Gold nanoparticles induce transcriptional activity of NF-κB in a B-lymphocyte cell line

Similar Documents

Hydrogen Peroxide Activates NF-κB through Tyrosine Phosphorylation of IκBα and Serine Phosphorylation of p65 EVIDENCE FOR THE INVOLVEMENT OF IκBα KINASE AND Syk PROTEIN-TYROSINE KINASE

Inhibition of Interleukin-1-stimulated NF-κB RelA/p65 Phosphorylation by Mesalamine Is Accompanied by Decreased Transcriptional Activity

Phosphorylation of Thr-516 and Ser-520 in the Kinase Activation Loop of MEKK3 Is Required for Lysophosphatidic Acid-mediated Optimal IκB Kinase β (IKKβ)/Nuclear Factor-κB (NF-κB) Activation

A20-binding Inhibitor of Nuclear Factor-κB (NF-κB)-2 (ABIN-2) Is an Activator of Inhibitor of NF-κB (IκB) Kinase α (IKKα)-mediated NF-κB Transcriptional Activity

Phosphorylation of RelA/p65 on Serine 536 Defines an IκBα-independent NF-κB Pathway

IκB Kinase β (IKBKB) Mutations in Lymphomas That Constitutively Activate Canonical Nuclear Factor κB (NFκB) Signaling

HSCARG inhibits activation of NF-κB by interacting with IκB kinase-β

IKKβ Is Required for Bcl-2-mediated NF-κB Activation in Ventricular Myocytes

3-Phosphoinositide-dependent Protein Kinase-1-mediated IκB Kinase β (IKKB) Phosphorylation Activates NF-κB Signaling

Gold nanoparticles induce transcriptional activity of NF-κB in a B-lymphocyte cell line