NDLI: Characterization of fullerene colloidal suspension in a cell culture medium for in vitro toxicity assessment

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Emergence and natural selection of drug-resistant prions

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Characterization of fullerene colloidal suspension in a cell culture medium for in vitro toxicity assessment

Beyond the bounds of orthology: functional inference from metagenomic context

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Characterization of fullerene colloidal suspension in a cell culture medium for in vitro toxicity assessment

Content Provider	Royal Society of Chemistry (RSC)
Author	Kato, Haruhisa Shinohara, Naohide Kinugasa, Shinichi Nakamura, Ayako Endoh, Shigehisa Horie, Masanori Fujita, Katsuhide Takahashi, Kayori Iwahashi, Hitoshi
Copyright Year	2010
Abstract	To elucidate important parameters for in vitro toxicity assessment of C60 and C70 fullerene colloidal particles, experiments were carried out in culture medium using pulsed field gradient nuclear magnetic resonance (PFG-NMR), asymmetrical flow field-flow fractionation (AFFFF), and dynamic light scattering (DLS) methods. First, the amounts of total and bulk bovine serum albumin (BSA) molecules in C60 and C70 fullerene colloidal suspensions were determined using the PFG-NMR and AFFFF methods. Because the amount of bulk BSA molecules in the cell culture medium is a significant factor in inducing cell growth and because BSA can strongly adsorb onto the fullerene particles, this value is an important parameter for toxicological assessment. It was found that most of the BSA molecules are freely diffusing for both fullerene colloidal suspensions, at least in the range of fullerene concentration from 0.0025–0.15 mg mL−1. Second, structural analysis of the fullerene colloidal nanoparticles was successfully performed using AFFFF-multi angle light scattering (MALS) and DLS methods. Based on the observed light scattering profiles obtained from a narrow size distribution of colloidal particles collected after AFFFF separation, it was estimated that the fullerene colloidal nanoparticles of both C60 and C70 did not adopt a hard spherical structure in the culture medium. The results from combined analysis using the AFFFF-MALS and DLS methods also supported this conclusion and indicated that the fullerene colloidal particles adopted a more flexible structure in culture medium. Since carbon nanomaterials with different geometric structures exhibit quite different cytotoxicity and bioactivity, these results are important for in vitro toxicity assessment.
Starting Page	1238
Ending Page	1246
Page Count	9
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	6
Issue Number	7
Journal	Molecular BioSystems
DOI	10.1039/c002364g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Field flow fractionation Bovine serum albumin Cell culture Scattering Nanomaterials Cytotoxicity Carbon Fullerene BSA Dynamic light scattering Pulsed field gradient Nuclear magnetic resonance C70 fullerene
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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BSA Bovine serum albumin

Abbreviations: BSA- Bovine Serum Albumin

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Characterization of fullerene colloidal suspension in a cell culture medium for in vitro toxicity assessment

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BSA Bovine serum albumin

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