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| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Zheng, Hua Quan, Chang-Yun Wu, De-Qun Zhuo, Ren-Xi Zhang, Xian-Zheng Lu, Bo |
| Copyright Year | 2010 |
| Abstract | Through incorporating lactobionic acid (LA) bearing a galactose group to N-succinyl-chitosan-graft-polyethylenimine (NSC-g-PEI), NSC-g-PEI-LA copolymers were synthesized as gene vectors with hepatocyte targeting properties. The molecular weight and composition of NSC-g-PEI-LA copolymers were characterized using gel permeation chromatography (GPC) and 1H nuclear magnetic resonance spectroscopy (1H NMR) respectively. Agarose gel electrophoresis assays showed good DNA binding ability of NSC-g-PEI-LA, and the particle size of the NSC-g-PEI-LA/DNA complexes were between 150 and 400 nm as determined by a Zeta sizer. The NSC-g-PEI-LA/DNA complexes observed by scanning electron microscopy (SEM) exhibited a compact and spherical morphology. The zeta potentials of these complexes were increased with the weight ratio of NSC-g-PEI-LA/DNA. NSC-g-PEI-LA has a lower cytotoxicity than PEI (25 kDa) and the toxicity decreased with increasing substitution of LA. The transfection efficiency of different complexes was evaluated by luciferase assay. Compared with PEI (25 kDa) and NSC-g-PEI/DNA, NSC-g-PEI-LA showed good transfection activity and cell specificity to HepG2 cells. The results suggested that NSC-g-PEI-LA has the potential to be used as a safe and effective targeting gene vector. |
| Starting Page | 2529 |
| Ending Page | 2538 |
| Page Count | 10 |
| File Format | HTM / HTML PDF |
| ISSN | 1742206X |
| Volume Number | 6 |
| Issue Number | 12 |
| Journal | Molecular BioSystems |
| DOI | 10.1039/c0mb00096e |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Open |
| Subject Keyword | Gel electrophoresis Euclidean vector Luciferase Nuclear magnetic resonance spectroscopy Cytotoxicity Scanning electron microscope Gel permeation chromatography Zeta Transfection Gene Hepatocyte LA DNA Los Angeles Nuclear magnetic resonance Galactose |
| Content Type | Text |
| Resource Type | Article |
| Subject | Molecular Biology Biotechnology |
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