NDLI: Galactosyl conjugated N-succinyl-chitosan-graft-polyethylenimine for targeting gene transfer

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Molecular BioSystems Vol - 6 Issue - 9

Molecular BioSystems Vol - 6 Issue - 8

Molecular BioSystems Vol - 6 Issue - 7

Molecular BioSystems Vol - 6 Issue - 6

Molecular BioSystems Vol - 6 Issue - 5

Molecular BioSystems Vol - 6 Issue - 4

Molecular BioSystems Vol - 6 Issue - 3

Molecular BioSystems Vol - 6 Issue - 2

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Galactosyl conjugated N-succinyl-chitosan-graft-polyethylenimine for targeting gene transfer

Content Provider	Royal Society of Chemistry (RSC)
Author	Zheng, Hua Quan, Chang-Yun Wu, De-Qun Zhuo, Ren-Xi Zhang, Xian-Zheng Lu, Bo
Copyright Year	2010
Abstract	Through incorporating lactobionic acid (LA) bearing a galactose group to N-succinyl-chitosan-graft-polyethylenimine (NSC-g-PEI), NSC-g-PEI-LA copolymers were synthesized as gene vectors with hepatocyte targeting properties. The molecular weight and composition of NSC-g-PEI-LA copolymers were characterized using gel permeation chromatography (GPC) and 1H nuclear magnetic resonance spectroscopy (1H NMR) respectively. Agarose gel electrophoresis assays showed good DNA binding ability of NSC-g-PEI-LA, and the particle size of the NSC-g-PEI-LA/DNA complexes were between 150 and 400 nm as determined by a Zeta sizer. The NSC-g-PEI-LA/DNA complexes observed by scanning electron microscopy (SEM) exhibited a compact and spherical morphology. The zeta potentials of these complexes were increased with the weight ratio of NSC-g-PEI-LA/DNA. NSC-g-PEI-LA has a lower cytotoxicity than PEI (25 kDa) and the toxicity decreased with increasing substitution of LA. The transfection efficiency of different complexes was evaluated by luciferase assay. Compared with PEI (25 kDa) and NSC-g-PEI/DNA, NSC-g-PEI-LA showed good transfection activity and cell specificity to HepG2 cells. The results suggested that NSC-g-PEI-LA has the potential to be used as a safe and effective targeting gene vector.
Starting Page	2529
Ending Page	2538
Page Count	10
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	6
Issue Number	12
Journal	Molecular BioSystems
DOI	10.1039/c0mb00096e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Gel electrophoresis Euclidean vector Luciferase Nuclear magnetic resonance spectroscopy Cytotoxicity Scanning electron microscope Gel permeation chromatography Zeta Transfection Gene Hepatocyte LA DNA Los Angeles Nuclear magnetic resonance Galactose
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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