NDLI: Development and application of a miniaturized gel electrophoresis device for protein analysis

Please wait, while we are loading the content...

Mismatch formation in solution and on DNA microarrays: how modified nucleosides can overcome shortcomings of imperfect hybridization caused by oligonucleotide composition and base pairing

Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivity

Correlation network analysis for data integration and biomarker selection

Development and application of a miniaturized gel electrophoresis device for protein analysis

Back matter

Back cover

Molecular BioSystems Vol - 4 Issue - 2

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Development and application of a miniaturized gel electrophoresis device for protein analysis

Content Provider	Royal Society of Chemistry (RSC)
Author	Franssila, Sami Shimmo, Masahiko Pöysä, Eemeli Saura, Susanna Baumann, Marc Demianova, Zuzana Ihalainen, Saara
Copyright Year	2008
Abstract	The present article describes a miniaturized polyacrylamide slab gel electrophoresis-chip (PASGE-Chip) that can rapidly separate a set of predefined samples as well as cell lysate samples for clinical diagnosis. The chip consists of a polymethyl methacrylate (PMMA) upper unit (25 × 30 × 10 mm, width × length × depth) with integrated buffer chambers, running electrodes and loading wells and a bottom unit comprising a silicon dioxide-coated silicon plate with embossed gel chamber (11 × 15 × 0.37 mm). This miniaturized device was designed to be fast, easy to use and cheap to produce. The polyacrylamide slab gel electrophoresis can be performed in less than 10 min with low voltage. The gel-to-gel repeatability is around 3.8%. The limit of detection is approx. 10 ng as determined by Coomassie staining of selected standard proteins, and corresponds to a 10-fold increase in sensitivity as compared with a common size PAGE analysis device (e.g. 10 × 7 cm). The device was successfully applied to peptide mass fingerprint analysis, protein sequencing and ultra-sensitive immunodetection, and the performance was compared to a commonly used regular PAGE device.
Starting Page	260
Ending Page	265
Page Count	6
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	4
Issue Number	3
Journal	Molecular BioSystems
DOI	10.1039/b716850k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Polyacrylamide gel electrophoresis Fingerprint Gel electrophoresis Poly(methyl methacrylate) Protein sequencing Peptide Coomassie Para-Methoxy-N-methylamphetamine
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Far infrared-assisted embossing and bonding of poly(methyl methacrylate) microfluidic chips

Gel electrolytes based on poly(vinylidenefluoride-co-hexafluoropropylene)/thermoplastic polyurethane/poly(methyl methacrylate) with in situ SiO₂ for polymer lithium batteries

Surface relaxations of poly(methyl methacrylate) assessed by friction force microscopy on the nanoscale

Synthesis of superparamagnetic Fe₃O₄/PMMA/SiO₂ nanorattles with periodic mesoporous shell for lysozyme adsorption

Cellulose decorated cavities on ultrathin films of PMMA

Preparation of free-standing thermosensitive composite gel particles incorporating ionic liquids

Whole genome expression profiling using DNA microarray for determining biocompatibility of polymeric surfaces

Laser photolytic studies of sensitizers for negative photoresists: 2,7-diazidofluorene in poly (methyl methacrylate) films

Photochemical behaviour of tethered dianthryl compounds in sol–gel glass

Development and application of a miniaturized gel electrophoresis device for protein analysis

Similar Documents

Far infrared-assisted embossing and bonding of poly(methyl methacrylate) microfluidic chips

Gel electrolytes based on poly(vinylidenefluoride-co-hexafluoropropylene)/thermoplastic polyurethane/poly(methyl methacrylate) with in situ SiO2 for polymer lithium batteries

Surface relaxations of poly(methyl methacrylate) assessed by friction force microscopy on the nanoscale

Synthesis of superparamagnetic Fe3O4/PMMA/SiO2 nanorattles with periodic mesoporous shell for lysozyme adsorption

Cellulose decorated cavities on ultrathin films of PMMA

Preparation of free-standing thermosensitive composite gel particles incorporating ionic liquids

Whole genome expression profiling using DNA microarray for determining biocompatibility of polymeric surfaces

Laser photolytic studies of sensitizers for negative photoresists: 2,7-diazidofluorene in poly (methyl methacrylate) films

Photochemical behaviour of tethered dianthryl compounds in sol–gel glass

Development and application of a miniaturized gel electrophoresis device for protein analysis

Gel electrolytes based on poly(vinylidenefluoride-co-hexafluoropropylene)/thermoplastic polyurethane/poly(methyl methacrylate) with in situ SiO₂ for polymer lithium batteries

Synthesis of superparamagnetic Fe₃O₄/PMMA/SiO₂ nanorattles with periodic mesoporous shell for lysozyme adsorption