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Poly-N-acetylglucosamine and poly(glycerol phosphate) teichoic acid identification from staphylococcal biofilm extracts using excitation sculptured TOCSY NMR

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Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Dynamic change in promoter activation during lysine biosynthesis in Escherichia coli cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Yamada, Tadashi Furusawa, Chikara Yomo, Tetsuya Hirasawa, Takashi Ou, Jianhong Nagahisa, Keisuke Shimizu, Hiroshi
Copyright Year	2008
Abstract	We investigated the expression dynamics of genes involved in lysine biosynthesis in Escherichia coli cells to obtain a quantitative understanding of the gene regulatory system. By constructing reporter strains expressing the green fluorescence protein (gfp) gene under the control of the promoter regions of those genes associated with lysine biosynthesis, time-dependent changes in gene expression in response to changes in lysine concentration in the medium were monitored by flow cytometry. Five promoters involved in lysine biosynthesis respond to the changes in lysine concentration in the medium. For these five promoters, time-dependent gene expression data were fitted to a simple dynamical model of gene expression to estimate the parameters of the gene regulatory system. According to the fitting parameters, dapD shows a significantly larger coefficient of repression than the other genes in the lysine synthesis pathway, which indicates the weak binding activity of the repressor to the dapDpromoter region. Moreover, there is a trend that the closer an enzyme is to the start of the lysine biosynthesis pathway, the smaller its maximal promoter activity is. The results provide a better quantitative understanding of the expression dynamics in the lysine biosynthesis pathway.
Starting Page	128
Ending Page	134
Page Count	7
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	4
Issue Number	2
Journal	Molecular BioSystems
DOI	10.1039/b711035a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Flow cytometry Reporter gene Gene Lysine Enzyme Escherichia coli Fluorescence Protein
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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