NDLI: Establishment of the platform for reverse chemical genetics targeting novel protein

Content Provider	Royal Society of Chemistry (RSC)
Author	Koga, Hisashi
Copyright Year	2006
Abstract	In the “drug discovery” era, protein–protein interaction modules are becoming the most exciting group of targets for study. Although combinatorial libraries and active natural products are rapidly and systemically being equipped by both for-profit and not-for-profit organizations, complete drug-screening systems have not been achieved. There is a growing need for the establishment of drug discovery assays for highly effective utilization of the collected small molecules on a large scale. To generate drug-screening systems, we plan to identify novel protein–protein interactions that may participate in human diseases. The interactions have been identified by MS/MS analysis following immunoprecipitation using antibodies prepared from our cDNA projects. The intracellular pathway involving the identified interaction is computationally constructed, which then clarifies its relationship to the candidate disease. The development of reverse chemical genetics based on such information should help us to realize a significant increment in the number of drug discovery assays available for use. In this article, I describe our strategy for drug discovery and then introduce the applicability of fluorescence intensity distribution analysis (FIDA) and the expression-ready constructs called “ORF trap clones” to reverse chemical genetics.
Starting Page	159
Ending Page	164
Page Count	6
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	2
Issue Number	3-4
Journal	Molecular BioSystems
DOI	10.1039/b517589e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Protein\u2013protein interaction Immunoprecipitation Complementary DNA Antibody Chemogenomics Drug discovery Combinatorics Open reading frame
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Reverse interactomics: decoding protein–protein interactions with combinatorial peptide libraries

A yeast display immunoprecipitation screen for targeted discovery of antibodies against membrane protein complexes.

Protein lethality investigated in terms of long range dynamical interactions

Predicting essential genes based on network and sequence analysis

Interaction and localization diversities of global and local hubs in human protein–protein interaction networks

Chemogenomics for drug discovery: clinical molecules from open access chemical probes.

Chemogenomics: drug discovery's panacea?

Tagged library approach facilitates forward chemical genetics

Targeting protein–protein interactions for cancer therapy

Establishment of the platform for reverse chemical genetics targeting novel protein–protein interactions

Similar Documents

Reverse interactomics: decoding protein–protein interactions with combinatorial peptide libraries

A yeast display immunoprecipitation screen for targeted discovery of antibodies against membrane protein complexes.

Protein lethality investigated in terms of long range dynamical interactions

Predicting essential genes based on network and sequence analysis

Interaction and localization diversities of global and local hubs in human protein–protein interaction networks

Chemogenomics for drug discovery: clinical molecules from open access chemical probes.

Chemogenomics: drug discovery's panacea?

Tagged library approach facilitates forward chemical genetics

Targeting protein–protein interactions for cancer therapy

Establishment of the platform for reverse chemical genetics targeting novel protein–protein interactions