NDLI: DNA thermodynamic stability and supercoil dynamics determine the gene expression program during the bacterial growth cycle

Content Provider	Royal Society of Chemistry (RSC)
Author	Sobetzko, Patrick Glinkowska, Monika Muskhelishvili, Georgi Travers, Andrew
Copyright Year	2013
Abstract	The chromosomal DNA polymer constituting the cellular genetic material is primarily a device for coding information. Whilst the gene sequences comprise the digital (discontinuous) linear code, physiological alterations of the DNA superhelical density generate in addition analog (continuous) three-dimensional information essential for regulation of both chromosome compaction and gene expression. Insight into the relationship between the DNA analog information and the digital linear code is of fundamental importance for understanding genetic regulation. Our previous study in the model organism Escherichia coli suggested that the chromosomal gene order and a spatiotemporal gradient of DNA superhelicity associated with DNA replication determine the growth phase-dependent gene transcription. In this study we reveal a general gradient of DNA thermodynamic stability correlated with the polarity of chromosomal replication and manifest in the spatiotemporal pattern of gene transcription during the bacterial growth cycle. Furthermore, by integrating the physical and dynamic features of the transcribed sequences with their functional content we identify spatiotemporal domains of gene expression encompassing different functions. We thus provide both an insight into the organisational principle of the bacterial growth program and a novel holistic methodology for exploring chromosomal dynamics.
Starting Page	1643
Ending Page	1651
Page Count	9
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	9
Issue Number	7
Journal	Molecular BioSystems
DOI	10.1039/c3mb25515h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Linear code DNA replication Gene DNA Escherichia coli Polymer Chromosome Model organism Spatiotemporal pattern
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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Gene order and chromosome dynamics coordinate spatiotemporal gene expression during the bacterial growth cycle.

Gene order and chromosome dynamics coordinate spatiotemporal gene expression during the bacterial growth cycle.

Dynamics of Escherichia coli Chromosome Segregation during (2007)

Gene order and chromosome dynamics coordinate spatiotemporal gene expression during the bacterial growth cycle

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Regulatory elements coordinating initiation of chromosome replication to the Escherichia coli cell cycle.

Gene transcription and chromosome replication in escherichia coli.

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DNA thermodynamic stability and supercoil dynamics determine the gene expression program during the bacterial growth cycle

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