NDLI: Role of block copolymer-micelle nanocomposites in dye–bovine serum albumin binding: a combined experimental and molecular docking study

Please wait, while we are loading the content...

Molecular BioSystems Vol -13

Molecular BioSystems Vol -12

Molecular BioSystems Vol -9

Molecular BioSystems Vol - 9 Issue - 12

Molecular BioSystems Vol - 9 Issue - 9

Molecular BioSystems Vol - 9 Issue - 8

Molecular BioSystems Vol - 9 Issue - 7

Molecular BioSystems Vol - 9 Issue - 6

Molecular BioSystems Vol - 9 Issue - 5

Molecular BioSystems Vol - 9 Issue - 4

Molecular BioSystems Vol - 9 Issue - 3

Molecular BioSystems Vol - 9 Issue - 2

Front cover

Contents list

Analysis of omics data with genome-scale models of metabolism

Protein co-evolution: how do we combine bioinformatics and experimental approaches?

Systematic analysis of histone modification readout

Early self-reproduction, the emergence of division mechanisms in protocells

Reconstruction and analysis of the genome-scale metabolic network of Candida glabrata

Dissection of the potential characteristic of miRNA–miRNA functional synergistic regulations

From time series to biological network regulations: an evolutionary approach

Biochemical measurements on single erythroid progenitor cells shed light on the combinatorial regulation of red blood cell production

Role of block copolymer-micelle nanocomposites in dye–bovine serum albumin binding: a combined experimental and molecular docking study

Minimum set of mutations needed to optimize cyan fluorescent proteins for live cell imaging

Dynamic and structural constraints in signal propagation by regulatory networks

Systems-level characterization and engineering of oxidative stress tolerance in Escherichia coli under anaerobic conditions

A cellular stress-directed bistable switch controls the crosstalk between autophagy and apoptosis

Quantitative metabolomic and lipidomic profiling reveals aberrant amino acid metabolism in type 2 diabetes

Back matter

Back cover

Molecular BioSystems Vol -8

Molecular BioSystems Vol -7

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Role of block copolymer-micelle nanocomposites in dye–bovine serum albumin binding: a combined experimental and molecular docking study

Content Provider	Royal Society of Chemistry (RSC)
Author	Manna, Anamika Chakravorti, Sankar
Copyright Year	2013
Abstract	The role of a nanocomposite (NC), composed of intercalation of the diblock copolymer polyethylene-b-polyethylene glycol (PE-b-PEG) with the anionic surfactant sodium dodecyl sulphate (SDS), on the binding characteristics of bovine serum albumin (BSA) with a dye (1,8-naphthalimide, NAPMD) compared to the interaction between the same players in aqueous solution has been examined comprehensively in this paper. Static quenching due to complex formation in both NC medium and in buffer solution has been inferred on the basis of considerable changes in the absorption spectra of BSA on addition of NAPMD, of which the interaction is found to be stronger in NC medium. Temperature dependent fluorescence data also confirm an effective static quenching and stronger binding of NAPMD with BSA in NC medium. Peptide chain unfolding and denaturing of BSA in NC medium have been confirmed from steady state and time-resolved emission and circular dichroism data. This exposes both the tyrosine and tryptophan moieties as a unique case. Increased energy transfer between NAPMD and the tryptophan residue in the unfolded form of BSA helps in the appearance of tyrosine fluorescence in NC medium by quenching the tryptophan band. Ionization of the hydroxyl group in the aromatic ring of the tyrosine residue by the PEG group present in the NC medium produces a downshift of the tyrosine fluorescence band. The use of site selective markers confirms that NAPMD is near tryptophan in Sudlow’s site I in NC medium and in buffer solution it is away from tryptophan in Sudlow’s site II. The theoretical docking studies also vindicate the results of binding of NAPMD with BSA in site I or site II in NC and buffer media, as observed from different emission experiments including the site selective markers study.
Starting Page	246
Ending Page	257
Page Count	12
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	9
Issue Number	2
Journal	Molecular BioSystems
DOI	10.1039/c2mb25368b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Tyrosine Bovine serum albumin Lauric acid Fluorescence Tryptophan Surfactant Buffer solution Circular dichroism Sudlow BSA Dye Hydroxy group Sodium NC Nanocomposite Copolymer NAPMD
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Characterization of the interaction between eupatorin and bovine serum albumin by spectroscopic and molecular modeling methods (2013).

Studies on the Interaction of Cefepime Hydrochloride with Bovine Serum Albumin by Fluorescence, Synchronous Fluorescence, Three-Dimensional Fluorescence and Circular Dichroism

Deciphering the binding patterns and conformation changes upon the bovine serum albumin–rosmarinic acid complex

Photoacids as a new fluorescence tool for tracking structural transitions of proteins: following the concentration-induced transition of bovine serum albumin.

Studies of the Interaction between BSA and a Plumeran Indole Alkaloid Isolated from the Stem Bark of Aspidosperma cylindrocarpon (Apocynaceae)

Circular Dichroism of Bovine Plasma Albumin-Dye complexes

Bovine and Human Serum Albumin Interactions with 3-Carboxyphenoxathiin Studied by Fluorescence and Circular Dichroism Spectroscopy

Effect of bovine serum albumin on the micellization of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) block copolymers in aqueous solutions by fluorescence spectroscopy

Investigation of Pazopanib and Human Serum Albumin Interaction Using Spectroscopic and Molecular Docking Approaches

Role of block copolymer-micelle nanocomposites in dye–bovine serum albumin binding: a combined experimental and molecular docking study

Similar Documents

Characterization of the interaction between eupatorin and bovine serum albumin by spectroscopic and molecular modeling methods (2013).

Studies on the Interaction of Cefepime Hydrochloride with Bovine Serum Albumin by Fluorescence, Synchronous Fluorescence, Three-Dimensional Fluorescence and Circular Dichroism

Deciphering the binding patterns and conformation changes upon the bovine serum albumin–rosmarinic acid complex

Photoacids as a new fluorescence tool for tracking structural transitions of proteins: following the concentration-induced transition of bovine serum albumin.

Studies of the Interaction between BSA and a Plumeran Indole Alkaloid Isolated from the Stem Bark of Aspidosperma cylindrocarpon (Apocynaceae)

Circular Dichroism of Bovine Plasma Albumin-Dye complexes

Bovine and Human Serum Albumin Interactions with 3-Carboxyphenoxathiin Studied by Fluorescence and Circular Dichroism Spectroscopy

Effect of bovine serum albumin on the micellization of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) block copolymers in aqueous solutions by fluorescence spectroscopy

Investigation of Pazopanib and Human Serum Albumin Interaction Using Spectroscopic and Molecular Docking Approaches

Role of block copolymer-micelle nanocomposites in dye–bovine serum albumin binding: a combined experimental and molecular docking study