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Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 1. High abundance proteins, acute phase proteins and peptidomics

Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 2. Label-free relative quantitative proteomics

Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 3. Untargeted metabolomics

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Molecular BioSystems Vol - 12 Issue - 8

Molecular BioSystems Vol - 12 Issue - 7

Molecular BioSystems Vol - 12 Issue - 6

Molecular BioSystems Vol -9

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Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 3. Untargeted metabolomics

Content Provider	Royal Society of Chemistry (RSC)
Author	Herzyk, Pawel Eckersall, P. David McNeilly, Tom N. Thomas, Funmilola Clara Tassi, Riccardo Burgess, Karl Mudaliar, Manikhandan Burchmore, Richard Zadoks, Ruth N.
Copyright Year	2016
Abstract	Intramammary infection leading to bovine mastitis is the leading disease problem affecting dairy cows and has marked effects on the milk produced by infected udder quarters. An experimental model of Streptococcus uberis mastitis has previously been investigated for clinical, immunological and pathophysiological alteration in milk, and has been the subject of peptidomic and quantitative proteomic investigation. The same sample set has now been investigated with a metabolomics approach using liquid chromatography and mass spectrometry. The analysis revealed over 3000 chromatographic peaks, of which 690 were putatively annotated with a metabolite. Hierarchical clustering analysis and principal component analysis demonstrated that metabolite changes due to S. uberis infection were maximal at 81 hours post challenge with metabolites in the milk from the resolution phase at 312 hours post challenge being closest to the pre-challenge samples. Metabolic pathway analysis revealed that the majority of the metabolites mapped to carbohydrate and nucleotide metabolism show a decreasing trend in concentration up to 81 hours post-challenge whereas an increasing trend was found in lipid metabolites and di-, tri- and tetra-peptides up to the same time point. The increase in these peptides coincides with an increase in larger peptides found in the previous peptidomic analysis and is likely to be due to protease degradation of milk proteins. Components of bile acid metabolism, linked to the FXR pathway regulating inflammation, were also increased. Metabolomic analysis of the response in milk during mastitis provides an essential component to the full understanding of the mammary gland's response to infection.
Starting Page	2762
Ending Page	2769
Page Count	8
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	12
Issue Number	9
Journal	Molecular BioSystems
DOI	10.1039/c6mb00289g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Metabolomics Mastitis Time point Streptococcus uberis Metabolic pathway Inflammation Hierarchical clustering Metabolism Chromatography Lipid Streptococcus Protease Nucleotide Carbohydrate Mammary gland Mass spectrometry Udder Principal component analysis
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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