NDLI: Response of CnrX from Cupriavidus metallidurans CH34 to nickel binding

Please wait, while we are loading the content...

Ni(ii) ions cleave and inactivate human alpha-1 antitrypsin hydrolytically, implicating nickel exposure as a contributing factor in pathologies related to antitrypsin deficiency

Modulating glyoxalase I metal selectivity by deletional mutagenesis: underlying structural factors contributing to nickel activation profiles

Novel insights into nickel import in Staphylococcus aureus: the positive role of free histidine and structural characterization of a new thiazolidine-type nickel chelator

Response of CnrX from Cupriavidus metallidurans CH34 to nickel binding

Synthesis of nickel–iron hydrogenase in Cupriavidus metallidurans is controlled by metal-dependent silencing and un-silencing of genomic islands

A new insight into the role of intracellular nickel levels for the stress response, surface properties and twitching motility by Haemophilus influenzae

Nickel-responsive regulation of two novel Helicobacter pylori NikR-targeted genes

Dynamic HypA zinc site is essential for acid viability and proper urease maturation in Helicobacter pylori

SlyD-dependent nickel delivery limits maturation of [NiFe]-hydrogenases in late-stationary phase Escherichia coli cells

Rhizobium leguminosarum HupE is a highly-specific diffusion facilitator for nickel uptake

Regulation of a nickel–cobalt efflux system and nickel homeostasis in a soil actinobacterium Streptomyces coelicolor

[NiFe]-hydrogenases revisited: nickel–carboxamido bond formation in a variant with accrued O₂-tolerance and a tentative re-interpretation of Ni-SI states

Back cover

Metallomics Vol - 7 Issue - 3

Metallomics Vol - 7 Issue - 2

Response of CnrX from Cupriavidus metallidurans CH34 to nickel binding

Content Provider	Royal Society of Chemistry (RSC)
Author	Petit-Härtlein, Isabelle Maillard, Antoine P. Covès, Jacques Schleuder, Grit Künnemann, Sandra Nies, Dietrich H. Volbeda, Anne Große, Cornelia Rosny, Eve de
Copyright Year	2015
Abstract	Resistance to high concentration of nickel ions is mediated in Cupriavidus metallidurans by the CnrCBA transenvelope efflux complex. Expression of the cnrCBA genes is regulated by the transmembrane signal transduction complex CnrYXH. Together, the metal sensor CnrX and the transmembrane antisigma factor CnrY control the availability of the extracytoplasmic function sigma factor CnrH. Release of CnrH from sequestration by CnrY at the cytoplasmic side of the membrane depends essentially on the binding of the agonist metal ion Ni(II) to the periplasmic metal sensor domain of CnrX. CnrH availability leads to transcription initiation at the promoters cnrYp and cnrCp and to the expression of the genes in the cnrYXHCBA nickel resistance determinant. The first steps of signal propagation by CnrX rely on subtle metal-dependent allosteric modifications. To study the nickel-mediated triggering process by CnrX, we have altered selected residues, F66, M123, and Y135, and explored the physiological consequences of these changes with respect to metal resistance, expression of a cnrCBA-lacZ reporter fusion and protein production. M123C- and Y135F-CnrXs have been further characterized in vitro by metal affinity measurements and crystallographic structure analysis. Atomic-resolution structures of metal-bound M123C- and Y135F-CnrXs showed that Ni(II) binds two of the three canonical conformations identified and that Ni(II) sensing likely proceeds by conformation selection.
Starting Page	622
Ending Page	631
Page Count	10
File Format	HTM / HTML PDF
ISSN	17565901
Volume Number	7
Issue Number	4
Journal	Metallomics
DOI	10.1039/c4mt00293h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Agonist Determinant Sigma factor Ion Conformational change Cupriavidus metallidurans Protein Signal transduction Claudian letters Reporter gene Allosteric regulation Metal Resistance Nickel
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Metal sensing and signal transduction by CnrX from Cupriavidus metallidurans CH34: role of the only methionine assessed by a functional, spectroscopic, and theoretical study

Biostructural analysis of the metal-sensor domain of CnrX from Cupriavidus metallidurans CH34

Interplay between seven secondary metal uptake systems is required for full metal resistance of Cupriavidus metallidurans

Deletion of the zupT gene for a zinc importer influences zinc pools in Cupriavidus metallidurans CH34

Heavy metal resistance in Cupriavidus metallidurans CH34 is governed by an intricate transcriptional network

The biological chemistry of the transition metal “transportome” of Cupriavidus metallidurans

ArsR arsenic-resistance regulatory protein from Cupriavidus metallidurans CH34

Transcriptional activation of MerR family promoters in Cupriavidus metallidurans CH34

Precipitation of Silver-Thiosulfate Complex and Immobilization of Silver by Cupriavidus metallidurans CH34

Response of CnrX from Cupriavidus metallidurans CH34 to nickel binding

Similar Documents

Metal sensing and signal transduction by CnrX from Cupriavidus metallidurans CH34: role of the only methionine assessed by a functional, spectroscopic, and theoretical study

Biostructural analysis of the metal-sensor domain of CnrX from Cupriavidus metallidurans CH34

Interplay between seven secondary metal uptake systems is required for full metal resistance of Cupriavidus metallidurans

Deletion of the zupT gene for a zinc importer influences zinc pools in Cupriavidus metallidurans CH34

Heavy metal resistance in Cupriavidus metallidurans CH34 is governed by an intricate transcriptional network

The biological chemistry of the transition metal “transportome” of Cupriavidus metallidurans

ArsR arsenic-resistance regulatory protein from Cupriavidus metallidurans CH34

Transcriptional activation of MerR family promoters in Cupriavidus metallidurans CH34

Precipitation of Silver-Thiosulfate Complex and Immobilization of Silver by Cupriavidus metallidurans CH34

Response of CnrX from Cupriavidus metallidurans CH34 to nickel binding