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The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis

The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis

Content Provider	Royal Society of Chemistry (RSC)
Author	Hernández, Luis E. Álvarez-Fernández, Ana Abadía, Javier Escobar, Carolina Carrasco-Gil, Sandra Sobrino-Plata, Juan
Copyright Year	2014
Abstract	Recent research efforts have highlighted the importance of glutathione (GSH) as a key antioxidant metabolite for metal tolerance in plants. Little is known about the mechanisms involved in stress due to mercury (Hg), one of the most hazardous metals to the environment and human health. To understand the implication of GSH metabolism for Hg tolerance, we used two γ-glutamylcysteine synthetase (γECS) Arabidopsis thaliana allele mutants (rax1-1 and cad2-1) and a phytochelatin synthase (PCS) mutant (cad1-3). The leaves of these mutants and of wild type (Col-0) were infiltrated with a solution containing Cd or Hg (0, 3 and 30 μM) and incubated for 24 and 48 h. The formation of phytochelatins (PCs) in the leaf extracts was followed by two different HPLC-based methods and occurred in Col-0, cad2-1 and rax1-1 plants exposed to Cd, whereas in the Hg treatments, PCs accumulated mainly in Col-0 and rax1-1, where Hg–PC complexes were also detected. ASA and GSH/GSSG levels increased under moderate metal stress conditions, accompanied by increased GSH reductase (GR) activity and expression. However, higher metal doses led to a decrease in the analysed parameters, and stronger toxic effects appeared with 30 μM Hg. The GSH concentration was significantly higher in rax1-1 (70% of Col-0) than in cad2-1 (40% of Col-0). The leaves of rax1-1 were less sensitive than cad2-1, in accordance with the greater expression of γECS in rax1-1. Our results underline the existence of a minimal GSH concentration threshold needed to minimise the toxic effects exerted by Hg.
Starting Page	356
Ending Page	366
Page Count	11
File Format	HTM / HTML PDF
ISSN	17565901
Volume Number	6
Issue Number	2
Journal	Metallomics
DOI	10.1039/c3mt00329a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Arabidopsis thaliana Allele Glutathione disulfide Methylmercury Gamma-L-Glutamyl-L-cysteine The Leaves Mutation Film speed Antioxidant Metabolism Phytochelatin Glutathione
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

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In vivo phytochelatins and Hg–phytochelatin complexes in Hg-stressed Brassica chinensis L.

Zn-bis-glutathionate is the best co-substrate of the monomeric phytochelatin synthase from the photosynthetic heavy metal-hyperaccumulator Euglena gracilis

The CTR/COPT-dependent copper uptake and SPL7-dependent copper deficiency responses are required for basal cadmium tolerance in A. thaliana

Plants prioritize phytochelatin synthesis during cadmium exposure even under reduced sulfate uptake caused by the disruption of SULTR1;2

Role of C-terminal Cys-rich Region of Phytochelatin Synthase in Tolerance to Cadmium Ion Toxicity

Glutathione degradation activity of γ-glutamyl peptidase 1 manifests its dual roles in primary and secondary sulfur metabolism in Arabidopsis.

Reactivity of γ‐glutamyl‐cysteine with intracellular and extracellular glutathione metabolic enzymes

Enhanced arsenic accumulation by engineered yeast cells expressing Arabidopsis thaliana phytochelatin synthase.

From cysteine to longer chain thiols: thermodynamic analysis of cadmium binding by phytochelatins and their fragments

The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis

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