NDLI: Phylogenomic analysis of Cation Diffusion Facilitator proteins uncovers Ni<sup>2+</sup>/Co<sup>2+</sup> transporters

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Metallomics Vol - 5 Issue - 9

Metallomics Vol - 5 Issue - 8

Metallomics Vol - 5 Issue - 7

Metallomics Vol - 5 Issue - 6

Metallomics Vol - 5 Issue - 5

Metallomics Vol - 5 Issue - 4

Metallomics Vol - 5 Issue - 3

Metallomics Vol - 5 Issue - 2

Phylogenomic analysis of Cation Diffusion Facilitator proteins uncovers Ni²⁺/Co²⁺ transporters

Content Provider	Royal Society of Chemistry (RSC)
Author	Vinuesa, Pablo Santos, Alejandro García-de los Tabche, Maria Luisa Cubillas, Ciro
Copyright Year	2013
Abstract	The ubiquitous Cation Diffusion Facilitator proteins (CDF) play a key role in maintaining the cellular homeostasis of essential metal ions. Previous neighbor-joining phylogenetic analysis classified CDF proteins into three substrate-defined groups: Zn2+, Fe2+/Zn2+ and Mn2+. These studies were unable to discern substrate-defined clades for Ni2+, Co2+, Cd2+ and Cu2+ transporters, despite their existence in this family. In this study we improved the accuracy of this previous functional classification using a phylogenomic approach based on a thorough maximum-likelihood phylogeny and the inclusion of recently characterized CDF transporters. The inference of CDF protein function predicted novel clades for Zn2+, Fe2+, Cd2+ and Mn2+. The Ni2+/Co2+ and Co2+ substrate specificities of two clades containing uncharacterized proteins were defined through the functional characterization of nepA and cepA metal inducible genes which independently conferred Ni2+ and Co2+ resistances to Rhizobium etli CFN42 and increased, respectively, Ni2+/Co2+ and Co2+ resistances to Escherichia coli. Neither NepA nor CepA confer Zn2+, Fe2+ and Mn2+ resistances. The ability of NepA to confer Ni2+/Co2+ resistance is dependent on clade-specific residues Asn88 and Arg197 whose mutations produce a non-functional protein.
Starting Page	1634
Ending Page	1643
Page Count	10
File Format	HTM / HTML PDF
ISSN	17565901
Volume Number	5
Issue Number	12
Journal	Metallomics
DOI	10.1039/c3mt00204g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Neighbor joining CDF Escherichia coli Cation diffusion facilitator Homeostasis Phylogenetics Rhizobium CepA Protein
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

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