NDLI: Identification in human urine and blood of a novel selenium metabolite, Se-methylselenoneine, a potential biomarker of metabolization in mammals of the naturally occurring selenoneine, by HPLC coupled to electrospray hybrid linear ion trap-orbital ion trap MS

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Metallomics Vol - 3 Issue - 4

Metallomics Vol - 3 Issue - 3

Metallomics Vol - 3 Issue - 2

Metallomics Vol -2

Metallomics Vol -1

Identification in human urine and blood of a novel selenium metabolite, Se-methylselenoneine, a potential biomarker of metabolization in mammals of the naturally occurring selenoneine, by HPLC coupled to electrospray hybrid linear ion trap-orbital ion trap MS

Content Provider	Royal Society of Chemistry (RSC)
Author	Pannier, Florence Klein, Marlène Bueno, Maïté Ouerdane, Laurent
Copyright Year	2011
Abstract	Speciation analysis of selenium in human urine allowed for the first time the identification of a novel selenium metabolite, Se-methylselenoneine. Despite a concentration at low ppb level, its characterization was achieved after sample purification by solid phase extraction (SPE) followed by the parallel coupling of the bidimensional RP/HILIC chromatography with ICP-MS and ESI-LTQ Orbitrap MS detection. To confirm its biological significance with regards to selenoneine, the recently discovered analog of ergothioneine, and to discard the possibility of sample preparation artifacts, a new method was developed to monitor its actual presence, as well as the occurrence of its sulfur and/or non-methylated analogs, in non-preconcentrated urine and blood samples of non-supplemented humans. It consisted in a HILIC ESI-MS3 method in high resolution mode (resolution 30 000 at m/z 400) with large isolation width windows for precursor ions. These two particular settings allowed respectively to keep observing the specific mass defect of selenium- and sulfur-containing molecules and to maintain the characteristic selenium pattern in product ions created through MSn fragmentations. As a result, all four metabolites were detected in blood and three of them in urine. Moreover, different ratios “methylated/non-methylated” were observed between urine and blood samples, which seemed to indicate their active metabolization. The analytical tool developed here will be of a great importance to further study the occurrence and the potential metabolic role in mammalian organelles, cells and fluids of these very particular and promising redox metabolites.
Starting Page	513
Ending Page	520
Page Count	8
File Format	HTM / HTML PDF
ISSN	17565901
Volume Number	3
Issue Number	5
Journal	Metallomics
DOI	10.1039/c0mt00060d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Urine Ergothioneine Solid phase extraction Metabolite Binding energy Redox Selenium Orbitrap Sulfur Speciation Chromatography
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

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