NDLI: Imaging metals in Caenorhabditis elegans

Content Provider	Royal Society of Chemistry (RSC)
Author	Karst, U. Bornhorst, J. Palinski, C. Sperling, M. Aschner, M. Schwerdtle, T.
Copyright Year	2017
Abstract	Systemic trafficking and storage of essential metal ions play fundamental roles in living organisms by serving as essential cofactors in various cellular processes. Thereby metal quantification and localization are critical steps in understanding metal homeostasis, and how their dyshomeostasis might contribute to disease etiology and the ensuing pathologies. Furthermore, the amount and distribution of metals in organisms can provide insight into their underlying mechanisms of toxicity and toxicokinetics. While in vivo studies on metal imaging in mammalian experimental animals are complex, time- and resource-consuming, the nematode Caenorhabditis elegans (C. elegans) provides a suitable comparative and complementary model system. Expressing homologous genes to those inherent to mammals, including those that regulate metal homeostasis and transport, C. elegans has become a powerful tool to study metal homeostasis and toxicity. A number of recent technical advances have been made in the development and application of analytical methods to visualize metal ions in C. elegans. Here, we briefly summarize key findings and challenges of the three main techniques and their application to the nematode, namely sensing fluorophores, microbeam synchrotron radiation X-ray fluorescence as well as laser ablation (LA) coupled to inductively coupled plasma-mass spectrometry (ICP-MS).
Starting Page	357
Ending Page	364
Page Count	8
File Format	HTM / HTML PDF
ISSN	17565901
Volume Number	9
Issue Number	4
Journal	Metallomics
DOI	10.1039/c6mt00265j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Toxicokinetics Caenorhabditis elegans Staurogyne elegans X-ray fluorescence LA Cofactor (biochemistry) Homeostasis Los Angeles Laser ablation Synchrotron radiation Microbeam
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

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